Furukawa H, Nakayasu K, Gotoh T, Watanabe Y, Takano T, Ishikawa T, Kanai A
Kanai Juntendo University, Department of Ophthalmology, Tokyo, Japan.
J Refract Surg. 1997 Aug;13(5 Suppl):S457-8. doi: 10.3928/1081-597X-19970801-20.
Tranilast (trade name Rizaben), an anti-allergic drug with anti-inflammatory effects, is thought to inhibit synthesis of extracellular matrix of fibroblasts through the suppression of TGF-beta. We evaluated the effect of topical tranilast on the subepithelial haze that developed after excimer laser keratectomy and its effect was compared with that of betamethasone eye drops
Excimer laser keratectomy (phototherapeutic keratectomy mode) was performed with the Nidek EC-5000 excimer laser on 16 rabbit corneas (eight rabbits). From the second postoperative day, topical 2% tranilast was instilled in the right eye and the control solution in the left eye, four times daily. Until the fourth week after the operation, we measured the densitometric values of scattered light intensity of the subepithelial haze with an anterior ocular analyzer, EAS-1000 (Nidek). At the fifth postoperative week, light and electron microscopy and immunohistochemistry with an antibody to TGF-beta were also performed.
Densitometric values of the subepithelial haze in the corneas treated with 2% tranilast were slightly less than those of the subepithelial haze in the control corneas. However, the values of the subepithelial haze in the betamethasone-treated corneas were significantly less than those in control corneas. Histochemical examinations revealed that topical tranilast had a small effect on the subepithelial haze after excimer laser keratectomy in rabbits.
Topical 0.1% betamethasone can limit the amount of subepithelial haze and tranilast may inhibit development of subepithelial haze by the suppression of TGF-beta.
曲尼司特（商品名丽科明）是一种具有抗炎作用的抗过敏药物，被认为可通过抑制转化生长因子-β（TGF-β）来抑制成纤维细胞细胞外基质的合成。我们评估了局部应用曲尼司特对准分子激光角膜切削术后形成的上皮下混浊的影响，并将其效果与倍他米松滴眼液进行比较。
使用Nidek EC-5000准分子激光对16只兔角膜（8只兔子）进行准分子激光角膜切削术（光治疗性角膜切削模式）。术后第二天起，右眼滴入2%曲尼司特局部用药，左眼滴入对照溶液，每日4次。直到术后第四周，我们使用眼前节分析仪EAS-1000（Nidek）测量上皮下混浊散射光强度的光密度值。术后第五周，还进行了光镜和电镜检查以及用TGF-β抗体进行免疫组织化学检查。
用2%曲尼司特治疗的角膜中上皮下混浊的光密度值略低于对照角膜中上皮下混浊的光密度值。然而，用倍他米松治疗的角膜中上皮下混浊的值明显低于对照角膜中的值。组织化学检查显示，局部应用曲尼司特对兔准分子激光角膜切削术后的上皮下混浊影响较小。
局部应用0.1%倍他米松可限制上皮下混浊的量，曲尼司特可能通过抑制TGF-β来抑制上皮下混浊的形成。
