Thom S B, Myers J S, Rapuano C J, Eagle R C, Siepser S B, Gomes J A
Cataract and Primary Eye Care Service, Wills Eye Hospital, Philadelphia, Pennsylvania 19107, USA.
J Cataract Refract Surg. 1997 Nov;23(9):1324-30. doi: 10.1016/s0886-3350(97)80110-1.
To determine the relationship between anti-transforming growth factor-beta (anti-TGF-beta) antibodies and the amount of corneal stromal haze after excimer laser photorefractive keratectomy (PRK).
Wills Eye Hospital, Philadelphia, Pennsylvania, USA.
Nineteen rabbits had bilateral PRK. Dichlorotriazinyl fluorescein was used to stain the exposed stroma; all rabbits were then treated with antibiotic ointment for 4 days. Ten rabbits were randomized to treatment with topical anti-TGF-beta1, -beta2, and -beta3 antibody 50 microg three times a day for 4 days; the others received diluent three times a day for 4 days. Stromal haze was graded weekly for 8 weeks on a 0 to 4+ scale. At the end of the study, all corneas were examined histopathologically.
All treated eyes developed appreciable haze. Seven control rabbits and one antibody-treated rabbit had an epithelial erosion (P = .00001). Antibody-treated rabbits had significantly less haze at 3, 4, and 5 weeks (right eyes) and 3, 4, 5, 7 and 8 weeks (left eyes) (P < .05). Histopathology and fluorescence microscopy showed subepithelial collagen deposition consistent with clinical haze.
Topical anti-TGF-beta antibody reduced stromal haze after PRK in the rabbit model and may be clinically beneficial in humans.
确定准分子激光屈光性角膜切削术（PRK）后抗转化生长因子-β（抗-TGF-β）抗体与角膜基质 haze 量之间的关系。
美国宾夕法尼亚州费城的威尔斯眼科医院。
19 只兔子接受双侧 PRK。用二氯三嗪基荧光素对暴露的基质进行染色；然后所有兔子用抗生素眼膏治疗 4 天。10 只兔子被随机分配接受局部抗-TGF-β1、-β2 和 -β3 抗体 50μg，每天 3 次，共 4 天；其余兔子接受稀释剂，每天 3 次共 4 天。在 8 周内每周对基质 haze 进行 0 至 4+级评分。研究结束时，对所有角膜进行组织病理学检查。
所有治疗眼均出现明显 haze。7 只对照兔子和 1 只抗体治疗兔子发生上皮糜烂（P = 0.00001）。抗体治疗的兔子在第 3、4 和 5 周（右眼）以及第 3、4、5、7 和 8 周（左眼）的 haze 明显较少（P < 0.05）。组织病理学和荧光显微镜检查显示上皮下胶原沉积与临床 haze 一致。
局部抗-TGF-β抗体可减少兔模型 PRK 后的基质 haze，可能对人类临床有益。
