通过RNA指纹识别揭示的海马体中与记忆相关的晚期基因。
Late memory-related genes in the hippocampus revealed by RNA fingerprinting.
作者信息
Cavallaro S, Meiri N, Yi C L, Musco S, Ma W, Goldberg J, Alkon D L
机构信息
Laboratory of Adaptive Systems, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9669-73. doi: 10.1073/pnas.94.18.9669.
Abstract
Although long-term memory is thought to require a cellular program of gene expression and increased protein synthesis, the identity of proteins critical for associative memory is largely unknown. We used RNA fingerprinting to identify candidate memory-related genes (MRGs), which were up-regulated in the hippocampus of water maze-trained rats, a brain area that is critically involved in spatial learning. Two of the original 10 candidate genes implicated by RNA fingerprinting, the rat homolog of the ryanodine receptor type-2 and glutamate dehydrogenase (EC 1.4.1.3), were further investigated by Northern blot analysis, reverse transcription-PCR, and in situ hybridization and confirmed as MRGs with distinct temporal and regional expression. Successive RNA screening as illustrated here may help to reveal a spectrum of MRGs as they appear in distinct domains of memory storage.
摘要
尽管长期记忆被认为需要一个细胞程序来进行基因表达和增加蛋白质合成，但对于联想记忆至关重要的蛋白质的具体身份在很大程度上仍不清楚。我们使用RNA指纹技术来识别候选记忆相关基因（MRGs），这些基因在水迷宫训练大鼠的海马体中上调，海马体是一个在空间学习中起关键作用的脑区。通过RNA指纹技术鉴定出的最初10个候选基因中的两个，即ryanodine受体2型的大鼠同源物和谷氨酸脱氢酶（EC 1.4.1.3），通过Northern印迹分析、逆转录PCR和原位杂交进一步研究，并被确认为具有不同时间和区域表达的MRGs。如此处所示的连续RNA筛选可能有助于揭示在记忆存储的不同领域中出现的一系列MRGs。
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本文引用的文献
1
PUROMYCIN EFFECT ON MEMORY FIXATION IN THE GOLDFISH.嘌呤霉素对金鱼记忆巩固的影响。
Science. 1964 Nov 13;146(3646):952-3. doi: 10.1126/science.146.3646.952.
2
Memory in mice as affected by intracerebral puromycin.脑内嘌呤霉素对小鼠记忆的影响
Science. 1963 Jul 5;141(3575):57-9. doi: 10.1126/science.141.3575.57.
3
Ibotenate Lesions of Hippocampus and/or Subiculum: Dissociating Components of Allocentric Spatial Learning.海马体和/或下托的异搏定损伤：分离以自我为中心的空间学习的组成部分。
Eur J Neurosci. 1990;2(12):1016-1028. doi: 10.1111/j.1460-9568.1990.tb00014.x.
4
Impaired hippocampal representation of space in CA1-specific NMDAR1 knockout mice.CA1区特异性NMDAR1基因敲除小鼠海马体的空间表征受损。
Cell. 1996 Dec 27;87(7):1339-49. doi: 10.1016/s0092-8674(00)81828-0.
5
Expression of the transcription factor Zif268 in the temporal cortex of monkeys during visual paired associate learning.视觉配对联想学习过程中猴子颞叶皮质中转录因子Zif268的表达。
Eur J Neurosci. 1996 Oct;8(10):2118-28. doi: 10.1111/j.1460-9568.1996.tb00733.x.
6
1-[[[5-(4-Nitrophenyl)-2-furanyl]methylene]imino]-2,4-imidazolidinedione (dantrolene), an inhibitor of intracellular Ca2+ mobilization, impairs avoidance performance and spatial memory in mice.1-[[[5-(4-硝基苯基)-2-呋喃基]亚甲基]亚氨基]-2,4-咪唑啉二酮（丹曲林），一种细胞内钙离子动员抑制剂，会损害小鼠的回避行为表现和空间记忆。
Biol Pharm Bull. 1996 Aug;19(8):1038-40. doi: 10.1248/bpb.19.1038.
7
A role for immediate-early transcription factors in learning and memory.即刻早期转录因子在学习与记忆中的作用。
Behav Genet. 1996 May;26(3):293-9. doi: 10.1007/BF02359385.
8
Geometric determinants of the place fields of hippocampal neurons.海马神经元位置场的几何决定因素。
Nature. 1996 May 30;381(6581):425-8. doi: 10.1038/381425a0.
9
Aspects of the search for neural mechanisms of memory.记忆神经机制的探索方面
Annu Rev Psychol. 1996;47:1-32. doi: 10.1146/annurev.psych.47.1.1.
10
Differential time courses of c-fos mRNA expression in hippocampal subfields following acquisition and recall testing in mice.小鼠在获得和回忆测试后海马亚区中c-fos mRNA表达的差异时间进程。
Brain Res Cogn Brain Res. 1995 Oct;2(4):269-75. doi: 10.1016/0926-6410(95)90018-7.
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