Yeh T-S, Jan Y-Y, Chiu C-T, Ho Y-B, Chen T-C, Lee K-F, Chan K-M, Hsu J-C, Hwang T-L, Chen M-F
Department of Surgery, Chang Gung Memorial Hospital, Chang Gung University, Taipei.
Gut. 2002 Nov;51(5):712-6. doi: 10.1136/gut.51.5.712.
The pancreatic cystic neoplasms, including solid pseudopapillary tumour (SPT), mucinous cystic neoplasm (MCN), and intraductal papillary mucin producing tumour (IPMT), have their characteristic clinicopathological features. A systematic investigation of oestrogen receptor (OR), progesterone receptor (PR), trefoil factor 1(TFF1), and epidermal growth factor and its receptor (EGF and EGFR) expressed in pancreatic cystic neoplasms and pancreatic ductal adenocarcinoma was determined to elucidate their corresponding sex and age predilection, cell origin, and pathway of malignant transformation.
Surgical specimens of SPT (n=10), MCN (n=12), IPMT (n=10), and ductal adenocarcinoma (n=20) were studied. The expression of OR, PR, TFF1, EGF, and EGFR were each determined in each disease entity using monoclonal antibodies by immunohistochemical method. The results were correlated with the clinicopathological data.
PR was expressed in all 10 SPT, whereas OR was expressed in none of 10 SPT. TFF1 was not or weakly expressed in SPT. Although EGF was strongly expressed in seven of 10 SPT, synchronous expression of EGF and its receptor was expressed in none of 10 SPT. Of the 12 MCN, six had PR expression in the stroma cells but not in the neoplastic epithelium, seven had a moderate or strong expression of TFF1, and 10 had no or weak EGFR expression, irrespective of their benigneity or malignancy. Synchronous expression of EGF and EGFR was observed in only one of 12 MCN. Among 10 IPMT, TFF1 and EGFR were moderately or strongly expressed in all six malignancies, whereas TFF1 and EGFR were not or weakly expressed in three of four benigneity. Of 20 ductal adenocarcinomas, TFF1 and EGFR were moderately or strongly expressed in 16 and 12, respectively. Synchronous expression of EGF and EGFR was observed in six of 10 IPMT and nine of 20 ductal adenocarcinoma, respectively.
PR was uniquely expressed in SPT, and OR and PR were expressed in stroma of MCN, reflecting their sex and age predilection. TFF1 expression was related to EGFR such as in IPMT and ductal adenocarcinoma, not related to EGFR such as in MCN, and not related to hormonal receptors such as in SPT. EGF and its receptor might play a part in the malignant transformation of IPMT and ductal adenocarcinoma, but not of SPT and MCN.
胰腺囊性肿瘤，包括实性假乳头状瘤（SPT）、黏液性囊性肿瘤（MCN）和导管内乳头状黏液性肿瘤（IPMT），具有其特征性的临床病理特征。本研究系统调查了雌激素受体（OR）、孕激素受体（PR）、三叶因子1（TFF1）以及表皮生长因子及其受体（EGF和EGFR）在胰腺囊性肿瘤和胰腺导管腺癌中的表达情况，以阐明它们相应的性别和年龄偏好、细胞起源以及恶性转化途径。
研究了SPT（n = 10）、MCN（n = 12）、IPMT（n = 10）和导管腺癌（n = 20）的手术标本。通过免疫组织化学方法，使用单克隆抗体分别检测每种疾病实体中OR、PR、TFF1、EGF和EGFR的表达。将结果与临床病理数据进行关联分析。
10例SPT均表达PR，而无一例表达OR。TFF1在SPT中不表达或弱表达。虽然10例SPT中有7例强表达EGF，但10例SPT中无一例同时表达EGF及其受体。12例MCN中，6例在基质细胞中表达PR，肿瘤上皮细胞中不表达；7例TFF1呈中度或强表达；10例EGFR无表达或弱表达，无论其为良性或恶性。12例MCN中仅1例同时表达EGF和EGFR。10例IPMT中，6例恶性肿瘤均中度或强表达TFF1和EGFR，4例良性肿瘤中有3例TFF1和EGFR不表达或弱表达。20例导管腺癌中，16例和12例分别中度或强表达TFF1和EGFR。10例IPMT和20例导管腺癌中分别有6例和9例同时表达EGF和EGFR。
PR仅在SPT中表达，OR和PR在MCN的基质中表达，反映了它们的性别和年龄偏好。TFF1的表达在IPMT和导管腺癌中与EGFR相关，在MCN中与EGFR无关，在SPT中与激素受体无关。EGF及其受体可能在IPMT和导管腺癌的恶性转化中起作用，但在SPT和MCN中不起作用。
