Fultz P N, Su L, May P, West J T
Department of Microbiology, University of Alabama School of Medicine, Birmingham, Alabama, 35294, USA.
Virology. 1997 Sep 1;235(2):271-85. doi: 10.1006/viro.1997.8705.
It has been postulated that dual infections of humans with human immunodeficiency virus (HIV) and human T-cell leukemia/lymphotropic virus (HTLV) may potentiate disease progression. Counterparts of both of these pathogenic human retroviruses have been identified in various simian species indigenous to Asia and Africa, including sooty mangabey monkeys (Cercocebus atys). Using peripheral blood mononuclear cells (PBMC) from a mangabey naturally infected with both SIV and STLV-I, T-cell lines were established and maintained continuously for more than 3 years; these cell lines harbored only a newly identified mangabey STLV-I(sm) or both STLV-I(sm) and the acutely lethal variant SIVsmmPBj14. The dually infected cell line (FEd-P14) was established by de novo infection of mangabey PBMC with SIVsmmPBj14. This cell line was characterized by multiple assays which showed that structural proteins encoded by both viruses were produced in large quantities, but that the predominant viral glycoprotein on the cell surface was the STLV-I(sm) Env. Unusual interactions of the two retroviral glycoproteins were suggested by the formation of syncytia between Raji and the FEd-P14 cells, but not between Raji and simian cells infected with only one retrovirus or human cells infected with HTLV-I. The STLV-I(sm) strain obtained from the sooty mangabey was transmitted to normal macaque and mangabey PBMC and was shown to be unique by sequencing of the entire env gene. STLV-I(sm) from this African species was more closely related to "cosmopolitan" HTLV-I strains than to the prototypic STLV-I from an Asian pig-tailed macaque. In vitro and in vivo studies of STLV-I(sm) and SIVsmm, both isolated from a naturally infected mangabey monkey, may provide insight into disease induction and manifestations associated with coinfection by their human counterparts.
据推测，人类同时感染人类免疫缺陷病毒（HIV）和人类T细胞白血病/淋巴瘤病毒（HTLV）可能会加速疾病进展。在亚洲和非洲的各种灵长类物种中已鉴定出这两种致病性人类逆转录病毒的对应病毒，包括乌黑白眉猴（Cercocebus atys）。利用来自自然感染了猴免疫缺陷病毒（SIV）和猴T细胞白血病病毒1型（STLV-I）的乌黑白眉猴的外周血单个核细胞（PBMC），建立了T细胞系并连续维持了3年多；这些细胞系仅携带新鉴定出的乌黑白眉猴STLV-I（sm）或同时携带STLV-I（sm）和急性致死性变异株SIVsmmPBj14。通过用SIVsmmPBj14对乌黑白眉猴PBMC进行重新感染，建立了双重感染细胞系（FEd-P14）。通过多种检测对该细胞系进行了表征，结果表明两种病毒编码的结构蛋白均大量产生，但细胞表面主要的病毒糖蛋白是STLV-I（sm）Env。Raji细胞与FEd-P14细胞之间形成了合胞体，这表明两种逆转录病毒糖蛋白之间存在异常相互作用，但Raji细胞与仅感染一种逆转录病毒的猴细胞或感染HTLV-I的人类细胞之间未形成合胞体。从乌黑白眉猴获得的STLV-I（sm）毒株被传播到正常猕猴和乌黑白眉猴的PBMC，并通过对整个env基因进行测序显示其具有独特性。来自这种非洲物种的STLV-I（sm）与“世界性”HTLV-I毒株的亲缘关系比与来自亚洲猪尾猕猴的原型STLV-I更近。对从自然感染的乌黑白眉猴中分离出的STLV-I（sm）和SIVsmm进行的体外和体内研究，可能有助于深入了解其人类对应病毒共同感染所导致的疾病诱导和表现。
