Capaccio P, Pruneri G, Carboni N, Pagliari A V, Buffa R, Neri A, Ottaviani A, Pignataro L
Clinica Otorinolaringoiatrica I, Università di Milano, Ospedale Maggiore, I.R.C.C.S., Italy.
J Laryngol Otol. 1997 Jul;111(7):622-6. doi: 10.1017/s0022215100138149.
The expression of cyclin D1 gene was investigated in 74 laryngeal squamous cell carcinomas (LSCCs) in order to determine its clinical and prognostic value. Overexpression of cyclin D1 was detected immunohistochemically using DCS6 monoclonal antibody on formalin-fixed, paraffin-embedded tissue sections. Cyclin D1 expression was detected in 22 of the 74 cases investigated (30 per cent), thirteen of which presented nodal metastases (59 per cent); of the patients without any detectable cyclin D1 protein expression, six presented nodal metastases (12 per cent). Cyclin D1 protein expression was found in five per cent of the specimens of normal mucosa, eight per cent of those with low-grade dysplasia and 20 per cent of those with high-grade dysplasia. A statistically significant association was found between cyclin D1 expression and the supraglottic site (p < 0.05), tumour extension (p < 0.001), the presence of lymph node metastases (p < 0.001), and advanced clinical stage (p < 0.001). Cyclin D1 expression analysis is an important tool in the selection of LSCC patients with an aggressive clinical course.
为了确定细胞周期蛋白D1基因的临床和预后价值，对74例喉鳞状细胞癌（LSCC）进行了该基因表达情况的研究。采用DCS6单克隆抗体，通过免疫组织化学方法在福尔马林固定、石蜡包埋的组织切片上检测细胞周期蛋白D1的过表达情况。在74例研究病例中，有22例检测到细胞周期蛋白D1表达（30%），其中13例出现淋巴结转移（59%）；在未检测到细胞周期蛋白D1蛋白表达的患者中，有6例出现淋巴结转移（12%）。在正常黏膜标本中，5%检测到细胞周期蛋白D1蛋白表达；在低度发育异常标本中，8%检测到该蛋白表达；在高度发育异常标本中，20%检测到该蛋白表达。发现细胞周期蛋白D1表达与声门上部位（p < 0.05）、肿瘤浸润范围（p < 0.001）、淋巴结转移情况（p < 0.001）以及临床分期较晚（p < 0.001）之间存在统计学上的显著关联。细胞周期蛋白D1表达分析是选择具有侵袭性临床病程的喉鳞状细胞癌患者的一项重要工具。
