Johnson M L, Redmer D A, Reynolds L P
Department of Animal and Range Sciences, North Dakota State University, Fargo 58105, USA.
Biol Reprod. 1997 Sep;57(3):588-96. doi: 10.1095/biolreprod57.3.588.
Uterine growth, morphology, and endometrial cell proliferation were evaluated in intact estrous (EST; Day 0 of the estrous cycle) or luteal (LUT; Day 10 of the estrous cycle) ewes or in ovariectomized ewes receiving no hormone (OVX); estradiol-17beta (OVX+E); progesterone for 8 (OVX+P8), 30 (OVX+P30), or 60 (OVX+P60) days; estradiol followed by progesterone (OVX+E+P); or progesterone followed by estradiol (OVX +P+E). Uterine weights were greater for EST than for LUT ewes, were reduced 2- to 4-fold in OVX ewes, and were increased in estradiol-treated (OVX+E, OVX+E+P, and OVX+P+E) ewes. The effects of estradiol treatment on uterine growth in OVX ewes were primarily on cellular hypertrophy rather than hyperplasia. Endometrial labeling index (LI) was greater for EST than for LUT ewes and was low in OVX ewes. Compared with OVX ewes, OVX+E and OVX+P+E ewes had increased LI for all endometrial tissues, whereas OVX+P8 and OVX+E+P ewes had increased LI only for luminal epithelium. These data will help us determine the mechanisms by which ovarian steroids regulate uterine growth in ewes.
对处于发情期（EST；发情周期第0天）或黄体期（LUT；发情周期第10天）的完整母羊，或未接受激素处理的去卵巢母羊（OVX）、接受雌二醇-17β（OVX+E）、接受8天（OVX+P8）、30天（OVX+P30）或60天（OVX+P60）孕酮处理、接受雌二醇后再接受孕酮处理（OVX+E+P）或接受孕酮后再接受雌二醇处理（OVX+P+E）的去卵巢母羊的子宫生长、形态及子宫内膜细胞增殖情况进行了评估。EST母羊的子宫重量大于LUT母羊，OVX母羊的子宫重量降低了2至4倍，而接受雌二醇处理（OVX+E、OVX+E+P和OVX+P+E）的母羊子宫重量增加。雌二醇处理对OVX母羊子宫生长的影响主要是细胞肥大而非增生。EST母羊的子宫内膜标记指数（LI）高于LUT母羊，而OVX母羊的该指数较低。与OVX母羊相比，OVX+E和OVX+P+E母羊所有子宫内膜组织的LI均增加，而OVX+P8和OVX+E+P母羊仅管腔上皮的LI增加。这些数据将有助于我们确定卵巢类固醇调节母羊子宫生长的机制。
