嗜热厌氧菌那不勒斯嗜热栖热菌的葡萄糖转运系统。
The glucose transport system of the hyperthermophilic anaerobic bacterium Thermotoga neapolitana.
作者信息
Galperin MY, Noll KM, Romano AH
机构信息
Department of Molecular and Cell Biology, The University of Connecticut, Storrs, Connecticut 06269-3125
出版信息
Appl Environ Microbiol. 1996 Aug;62(8):2915-8. doi: 10.1128/aem.62.8.2915-2918.1996.
Abstract
The glucose transport system of the extremely thermophilic anaerobic bacterium Thermotoga neapolitana was studied with the nonmetabolizable glucose analog 2-deoxy-D-glucose (2-DOG). T. neapolitana accumulated 2-DOG against a concentration gradient in an intracellular free sugar pool that was exchangeable with external source of energy, such as pyruvate, and was inhibited by arsenate and gramicidin D. There was no phosphoenolpyruvate-dependent phosphorylation of glucose, 2-DOG, or fructose by cell extracts or toluene-treated cells, indicating the absence of a phosphoenolpyruvate:sugar phosphotransferase system. These data indicate that D-glucose is taken up by T. neapolitana via an active transport system that is energized by an ion gradient generated by ATP, derived from substrate-level phosphorylation.
摘要
利用不可代谢的葡萄糖类似物2-脱氧-D-葡萄糖（2-DOG）对嗜热厌氧菌那不勒斯嗜热栖热菌的葡萄糖转运系统进行了研究。那不勒斯嗜热栖热菌在细胞内游离糖池中逆浓度梯度积累2-DOG，该糖池可与外部能量源（如丙酮酸）进行交换，并受到砷酸盐和短杆菌肽D的抑制。细胞提取物或经甲苯处理的细胞对葡萄糖、2-DOG或果糖均未发生磷酸烯醇式丙酮酸依赖性磷酸化，表明不存在磷酸烯醇式丙酮酸：糖磷酸转移酶系统。这些数据表明，那不勒斯嗜热栖热菌通过一种主动转运系统摄取D-葡萄糖，该转运系统由底物水平磷酸化产生的ATP所产生的离子梯度提供能量。
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