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脲酶诱导合成尿液和人体尿液中磷酸钙和磷酸镁铵的结晶。

Urease-induced crystallizations of calcium phosphate and magnesium ammonium phosphate in synthetic urine and human urine.

作者信息

Ebisuno S, Komura T, Yamagiwa K, Ohkawa T

机构信息

Division of Urology, Minami Wakayama Hospital, Tanabe shi, Japan.

出版信息

Urol Res. 1997;25(4):263-7. doi: 10.1007/BF00942096.

DOI:10.1007/BF00942096
PMID:9286035
Abstract

An aggregometer technique was used to study urease-induced crystallizations in synthetic urine and human urine from healthy subjects and patients with chronic spinal cord injuries. The two different phases of crystallization, calcium phosphate and magnesium ammonium phosphate, were easily evaluated with a single assay using this technique. The crystallization of calcium phosphate and magnesium ammonium phosphate varied markedly among the different urine specimens after incubation with urease. The turbidity curves from human urine were divided into four patterns. We assumed that the variations in the patterns of the turbidity curves appeared to be mainly due to differences in the composition of the urine and in the original pH, and that the calcium and magnesium concentrations were very important in the urinary constituents.

摘要

采用凝聚仪技术研究脲酶诱导的合成尿液以及健康受试者和慢性脊髓损伤患者的人尿液中的结晶情况。使用该技术通过单一测定法就能轻松评估结晶的两个不同阶段,即磷酸钙和磷酸镁铵。与脲酶孵育后,不同尿液标本中磷酸钙和磷酸镁铵的结晶情况差异显著。人尿液的浊度曲线分为四种模式。我们认为,浊度曲线模式的差异似乎主要归因于尿液成分和初始pH值的不同,而且钙和镁的浓度在尿液成分中非常重要。

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本文引用的文献

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Effect of urinary macromolecules on aggregation of calcium oxalate in recurrent calcium stone formers and healthy.尿大分子对复发性草酸钙结石患者和健康人草酸钙聚集的影响。
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Urinary macromolecules are promoters of calcium oxalate nucleation in human urine: turbidimetric studies.
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Urease-induced crystallization in synthetic urine.脲酶诱导合成尿液中的结晶。
J Urol. 1985 Mar;133(3):529-32. doi: 10.1016/s0022-5347(17)49047-1.
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J Urol. 1986 Feb;135(2):416-9. doi: 10.1016/s0022-5347(17)45655-2.
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The effects of urease in undiluted human urine.未稀释人尿中脲酶的作用。
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7
Inhibition of calcium oxalate monohydrate crystal aggregation by urine proteins.尿蛋白对一水合草酸钙晶体聚集的抑制作用。
Am J Physiol. 1989 Jul;257(1 Pt 2):F99-106. doi: 10.1152/ajprenal.1989.257.1.F99.
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Urol Res. 1990;18(6):413-7. doi: 10.1007/BF00297375.
9
Studies of urease-induced crystallisation in undiluted human urine using the Coulter counter technique.
Urol Res. 1991;19(3):171-5. doi: 10.1007/BF00303744.
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The influence of pH and urine composition on urease enzymatic activity in human urine.
Urol Res. 1992;20(1):35-9. doi: 10.1007/BF00294332.