Potocnik A J, Kohler H, Eichmann K
Max Planck Institute for Immunobiology, Stübeweg 51, 79108 Freiburg, Germany.
Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10295-300. doi: 10.1073/pnas.94.19.10295.
During differentiation in vitro, embryonic stem (ES) cells generate progenitors for most hemato-lymphoid lineages. We studied the developmental potential of two ES cell subpopulations that share the fetal stem cell antigen AA4.1 but differ in expression of the lymphoid marker B220 (CD45R). Upon transfer into lymphoid deficient mice, the B220+ population generated a single transient wave of IgM+ IgD+ B cells but failed to generate T cells. In contrast, transfer of the B220- fraction achieved long-term repopulation of both T and B lymphoid compartments and restored humoral and cell-mediated immune reactions in the recipients. To assess the hemato-lymphopoietic potential of ES cell subsets in comparison to their physiological counterparts, cotransplantation experiments with phenotypically homologous subsets of fetal liver cells were performed, revealing a more potent developmental capacity of the latter. The results suggest that multipotential and lineage-committed lymphoid precursors are generated during in vitro differentiation of ES cells and that both subsets can undergo complete final maturation in vivo.
在体外分化过程中，胚胎干细胞（ES细胞）可生成大多数血液淋巴细胞谱系的祖细胞。我们研究了两个ES细胞亚群的发育潜能，这两个亚群均表达胎儿干细胞抗原AA4.1，但淋巴样标志物B220（CD45R）的表达不同。将其移植到缺乏淋巴细胞的小鼠体内后，B220+亚群产生了一波短暂的IgM+IgD+B细胞，但未能产生T细胞。相比之下，移植B220-亚群可实现T和B淋巴细胞区室的长期重建，并恢复受体的体液免疫和细胞介导的免疫反应。为了评估ES细胞亚群与它们的生理对应物相比的血液淋巴细胞生成潜能，我们进行了与胎儿肝细胞表型同源亚群的共移植实验，结果显示后者具有更强的发育能力。结果表明，在ES细胞的体外分化过程中会产生多能性和定向分化的淋巴样前体细胞，并且这两个亚群在体内均可经历完全的最终成熟。
