Keller G M
National Jewish Center for Immunology and Respiratory Medicine, Denver, USA.
Curr Opin Cell Biol. 1995 Dec;7(6):862-9. doi: 10.1016/0955-0674(95)80071-9.
Under appropriate conditions in culture, embryonic stem cells will differentiate and form embryoid bodies that have been shown to contain cells of the hematopoietic, endothelial, muscle and neuronal lineages. Many aspects of the lineage-specific differentiation programs observed within the embryoid bodies reflect those found in the embryo, indicating that this model system provides access to early cell populations that develop in a normal fashion. Recent studies involving the differentiation of genetically altered embryonic stem cells highlight the potential of this in vitro differentiation system for defining the function of genes in early development.
在适当的培养条件下，胚胎干细胞会分化并形成胚状体，已证明这些胚状体含有造血、内皮、肌肉和神经谱系的细胞。在胚状体内观察到的谱系特异性分化程序的许多方面都反映了胚胎中的情况，这表明该模型系统能够获取以正常方式发育的早期细胞群体。最近涉及基因改造胚胎干细胞分化的研究突出了这种体外分化系统在确定基因在早期发育中功能方面的潜力。
