Smith M P, Keay S D, Hall L, Harlow C R, Jenkins J M
University of Bristol, Division of Obstetrics and Gynaecology, St. Michael's Hospital, UK.
Mol Hum Reprod. 1997 Aug;3(8):651-4. doi: 10.1093/molehr/3.8.651.
The activity of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) in human granulosa cells has been shown to be associated with the outcome of treatment following in-vitro fertilization and embryo transfer. There are two known isoforms of 11 beta-HSD which differ significantly in their actions and co-factor requirements. The net activity of 11 beta-HSD which differ significantly in their actions and co-factor requirements. The net activity of 11 beta-HSD within the human ovary is unclear, but may be of particular importance within the ovarian follicle in regulating possible glucocorticoid influences on the oocyte. This study presents preliminary information regarding establishment of techniques to identify transcripts of the 11 beta-HSD isoforms within human granulosa cells and human cumulus cells using reverse transcription-polymerase chain reaction. In view of the high expression of the type 1 11 beta-HSD isoform and the possibility of other 11 beta-HSD isoforms in the ovary, plasmid technology was used to confirm the technique specifically identifying the known isoforms.
11β-羟基类固醇脱氢酶（11β-HSD）在人颗粒细胞中的活性已被证明与体外受精和胚胎移植后的治疗结果相关。已知11β-HSD有两种同工型，它们在作用和辅助因子需求方面有显著差异。11β-HSD的净活性在作用和辅助因子需求方面有显著差异。人卵巢内11β-HSD的净活性尚不清楚，但在调节糖皮质激素对卵母细胞可能的影响方面，卵巢卵泡内的11β-HSD可能尤为重要。本研究提供了关于使用逆转录-聚合酶链反应来鉴定人颗粒细胞和人卵丘细胞中11β-HSD同工型转录本的技术建立的初步信息。鉴于1型11β-HSD同工型在卵巢中的高表达以及卵巢中存在其他11β-HSD同工型的可能性，采用质粒技术来证实该技术可特异性鉴定已知的同工型。
