Miyahara S, Komori T, Fujiwara R, Shizuya K, Yamamoto M, Ohmori M, Okazaki Y
Department of Psychiatry, Mie University School of Medicine, Tsu, Japan.
Life Sci. 2000;66(6):PL93-8. doi: 10.1016/s0024-3205(99)00626-8.
We examined the effects of single and repeated stress on the expression of interleukin-6 (IL-6) and IL-6 receptor (IL-6R) mRNAs in the rat midbrain and hypothalamus using reverse transcriptase-polymerase chain reaction (RT-PCR). Following a single episode of restraint stress for 4 hours (1R) or 4 hours per day on two (2R) or three (3R) consecutive days, the hypothalamus and midbrain were removed immediately and the levels of IL-6 and IL-6R mRNAs in both regions were determined. Regional differences in stress-related changes in mRNA levels were noted. The expression of IL-6 mRNA in the hypothalamus did not change in 1R group but decreased in 2R and 3R groups. The expression of IL-6R mRNA in the same region significantly diminished in all groups. In the midbrain, the expression of IL-6 mRNA increased in 1R group and decreased in 2R and 3R, while the expression of IL-6R mRNA significantly diminished in 1R and 3R groups but was not different from control in 2R group. Our findings indicate that repeated stress in rats produce changes in IL-6 and IL-6R mRNAs in the midbrain and hypothalamus that are different than those of a single stress episode.
我们使用逆转录聚合酶链反应（RT-PCR）研究了单次和重复应激对大鼠中脑和下丘脑白细胞介素-6（IL-6）及白细胞介素-6受体（IL-6R）mRNA表达的影响。在单次约束应激4小时（1R）或连续两天（2R）或三天（3R）每天应激4小时后，立即取出下丘脑和中脑，测定这两个区域中IL-6和IL-6R mRNA的水平。注意到mRNA水平与应激相关变化存在区域差异。下丘脑IL-6 mRNA的表达在1R组未发生变化，但在2R组和3R组中降低。同一区域IL-6R mRNA的表达在所有组中均显著减少。在中脑，IL-6 mRNA的表达在1R组增加，在2R组和3R组减少，而IL-6R mRNA的表达在1R组和3R组显著减少，但在2R组与对照组无差异。我们的研究结果表明，大鼠的重复应激会使中脑和下丘脑的IL-6及IL-6R mRNA发生变化，这些变化与单次应激事件不同。
