Vohradský J, Li X M, Thompson C J
Czech Academy of Sciences, Institute of Microbiology, Prague, Czech Republic.
Electrophoresis. 1997 Aug;18(8):1418-28. doi: 10.1002/elps.1150180817.
Multivariate statistical comparisons of two-dimensional protein (2-D) gel patterns were used for the first time to define stages of a biological developmental system. The differentiating procaryote, Streptomyces coelicolor, was radiolabeled in liquid cultures at 16 intervals during development, and radioactive proteins were separated and quantified on 2-D gels. Cluster, principal component, and correlation analyses classified these gel patterns into four distinct groups, each reflecting a pattern of gene expression specific for a stage of development. These studies focused our attention on a phase of arrested growth as a key regulatory transition leading to secondary metabolism and a phase of renewed growth. Proteins whose synthesis was switched on or off during the "transitional" phase (some 21 and 18, respectively) were identified and will be the focus of future studies designed to identify their physiological or regulatory function.
二维蛋白质（2-D）凝胶图谱的多变量统计比较首次被用于定义生物发育系统的阶段。在发育过程中的16个时间间隔点，对分化中的原核生物天蓝色链霉菌进行液体培养放射性标记，放射性蛋白质在二维凝胶上进行分离和定量。聚类分析、主成分分析和相关性分析将这些凝胶图谱分为四个不同的组，每组反映了特定发育阶段的基因表达模式。这些研究使我们将注意力集中在停滞生长阶段，这是导致次级代谢的关键调控转变阶段，以及重新生长阶段。确定了在“过渡”阶段合成开启或关闭的蛋白质（分别约为21种和18种），这些蛋白质将成为未来旨在确定其生理或调控功能研究的重点。
