细菌细胞周期的蛋白质组学分析。
Proteomic analysis of the bacterial cell cycle.
作者信息
Grünenfelder B, Rummel G, Vohradsky J, Röder D, Langen H, Jenal U
机构信息
Division of Molecular Microbiology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
出版信息
Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4681-6. doi: 10.1073/pnas.071538098. Epub 2001 Apr 3.
A global approach was used to analyze protein synthesis and stability during the cell cycle of the bacterium Caulobacter crescentus. Approximately one-fourth (979) of the estimated C. crescentus gene products were detected by two-dimensional gel electrophoresis, 144 of which showed differential cell cycle expression patterns. Eighty-one of these proteins were identified by mass spectrometry and were assigned to a wide variety of functional groups. Pattern analysis revealed that coexpression groups were functionally clustered. A total of 48 proteins were rapidly degraded in the course of one cell cycle. More than half of these unstable proteins were also found to be synthesized in a cell cycle-dependent manner, establishing a strong correlation between rapid protein turnover and the periodicity of the bacterial cell cycle. This is, to our knowledge, the first evidence for a global role of proteolysis in bacterial cell cycle control.
采用全局方法分析新月柄杆菌细胞周期中的蛋白质合成与稳定性。通过二维凝胶电泳检测到约四分之一（979个）估计的新月柄杆菌基因产物，其中144个呈现出不同的细胞周期表达模式。这些蛋白质中有81个通过质谱鉴定，并被归类到各种各样的功能组。模式分析表明共表达组在功能上是聚类的。在一个细胞周期过程中，共有48种蛋白质被快速降解。这些不稳定蛋白质中超过一半也被发现以细胞周期依赖性方式合成，这在快速蛋白质周转与细菌细胞周期的周期性之间建立了很强的相关性。据我们所知，这是蛋白质水解在细菌细胞周期控制中具有全局作用的首个证据。
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