Jusa E R, Inaba Y, Kono M, Asagoe T, Uwatoko K
Department of Veterinary Epizootiology, College of Bioresource Sciences, Nihon University, Kanagawa, Japan.
Res Vet Sci. 1997 May-Jun;62(3):261-4. doi: 10.1016/s0034-5288(97)90201-0.
Heparin inhibited haemagglutination by porcine reproductive and respiratory syndrome virus (PRRSV) and by Aujesky's disease virus, but failed to inhibit haemagglutination by parainfluenza virus type 3. The minimal inhibitory concentration of heparin required to inhibit 8 HA U of PRRSV haemagglutinin ranged from 0.1 to 1 U ml-1. Mouse erythrocytes failed to combine with the haemagglutination inhibitory factor of heparin. However, mouse erythrocytes treated with heparinase had greatly reduced agglutinability by PRRSV. The formation of a haemagglutinin-heparin complex could be observed by sedimenting heparin with the haemagglutinin. All these findings suggest that a heparin-like molecule on the surface of mouse erythrocytes serves as the virus-cell receptor.
肝素可抑制猪繁殖与呼吸综合征病毒（PRRSV）和奥杰斯基氏病病毒的血凝反应，但不能抑制3型副流感病毒的血凝反应。抑制8个血凝单位（HA U）PRRSV血凝素所需的肝素最小抑制浓度范围为0.1至1 U/ml。小鼠红细胞不能与肝素的血凝抑制因子结合。然而，用肝素酶处理过的小鼠红细胞被PRRSV凝集的能力大大降低。通过将肝素与血凝素一起沉淀，可以观察到血凝素-肝素复合物的形成。所有这些发现表明，小鼠红细胞表面的一种类肝素分子充当病毒-细胞受体。
