Perron R J, Eyre P
Can J Physiol Pharmacol. 1979 Nov;57(11):1316-20. doi: 10.1139/y79-196.
Sensitized bovine granulocytes release histamine when exposed to specific antigens. A unique modulation of histamine release by adrenergic agents has been shown in the bovine; beta-adrenergic agonists enhance and alpha-adrenergic agonists inhibit histamine release. This is an opposite response to that reported in other species. The present study was undertaken to determine the possible relationship between cyclic nucleotides and adrenergic agents in this species. Dibutyryl cAMP enhanced antigen-induced histamine release over the complete concentration range tested (10(-6)--10(-3)M); it also overcame, in a dose-dependent manner, the inhibition of antigen-induced histamine release produced by 10(-4) M phenylephrine. The 8-bromo cGMP AND 0-MONOBUTYRYL CGMP had no significant effect on antigen-induced histamine release nor did 8-bromo-cGMP have any significant effect on the enhancement of histamine release produced by 10(-4) M dibutyryl cAMP. These findings suggest that only cAMP has a role in the modulation of antigen-induced histamine release from bovine granulocytes.
致敏的牛粒细胞在接触特定抗原时会释放组胺。在牛身上已显示出肾上腺素能药物对组胺释放有独特的调节作用；β-肾上腺素能激动剂增强组胺释放，而α-肾上腺素能激动剂抑制组胺释放。这与其他物种报道的反应相反。本研究旨在确定该物种中环状核苷酸与肾上腺素能药物之间的可能关系。在测试的整个浓度范围（10⁻⁶ - 10⁻³M）内，二丁酰环磷酸腺苷（dibutyryl cAMP）增强了抗原诱导的组胺释放；它还以剂量依赖的方式克服了10⁻⁴M去氧肾上腺素对抗原诱导的组胺释放的抑制作用。8-溴环磷酸鸟苷（8-bromo cGMP）和0-单丁酰环磷酸鸟苷（0-MONOBUTYRYL CGMP）对抗原诱导的组胺释放没有显著影响，8-溴环磷酸鸟苷对10⁻⁴M二丁酰环磷酸腺苷产生的组胺释放增强也没有显著影响。这些发现表明，只有环磷酸腺苷（cAMP）在调节牛粒细胞抗原诱导的组胺释放中起作用。
