Krivorutchenko Y L, Andronovskaja I B, Hinkula J, Krivoshein Y S, Ljungdahl-Ståhle E, Pertel S S, Grishkovets V I, Zemlyakov A E, Wahren B
Department of Microbiology and Virology, Crimean Medical Institute, Ukraine.
Vaccine. 1997 Aug-Sep;15(12-13):1479-86. doi: 10.1016/s0264-410x(97)00065-0.
Muramyl dipeptide (MDP), eight new lipophilic MDP derivatives (MDPs) and three purified saponins were evaluated for their ability to induce immune responses in mice immunized with HIV-1 envelope protein rgp160 and for their ability to influence the HIV-1 replication in vitro. Three of nine new synthetic MDP derivatives (beta-butyl-MDP, MTPO-26 and beta-cholesteryl-MDP) and one saponin (Taurosid I) have been shown to induce strong humoral immune responses to HIV-1 envelope glycoproteins rgp160 and rgp120. Three substances (beta-butyl-MDP, MDP-cholyl and beta-G27-MDP) induced high levels of T-cell stimulation to HIV-1 rgp160. beta-butyl-MDP induced the strongest B- and T-cell responses to HIV-1 glycoproteins. Two substances (beta-butyl-MDP and Taurosid I) did not induce an enhancement of HIV-1 replication in vitro and can be considered as promising adjuvants.
对胞壁酰二肽（MDP）、8种新型亲脂性MDP衍生物（MDPs）和3种纯化皂苷进行了评估，考察它们在用HIV-1包膜蛋白rgp160免疫的小鼠中诱导免疫反应的能力，以及它们在体外影响HIV-1复制的能力。9种新型合成MDP衍生物中的3种（β-丁基-MDP、MTPO-26和β-胆固醇基-MDP）和1种皂苷（牛磺皂草苷I）已被证明可诱导对HIV-1包膜糖蛋白rgp160和rgp120产生强烈的体液免疫反应。3种物质（β-丁基-MDP、MDP-胆酰基和β-G27-MDP）对HIV-1 rgp160诱导高水平的T细胞刺激。β-丁基-MDP对HIV-1糖蛋白诱导最强的B细胞和T细胞反应。2种物质（β-丁基-MDP和牛磺皂草苷I）在体外未诱导HIV-1复制增强，可被视为有前景的佐剂。
