Squires E J, Lundström K
Department of Animal and Poultry Science, University of Guelph, Canada.
J Anim Sci. 1997 Sep;75(9):2506-11. doi: 10.2527/1997.7592506x.
The relationships between levels of skatole and androstenone in fat, plasma levels of skatole metabolites, plasma testosterone and estrone sulfate, and levels of cytochrome P450IIE1 in liver were investigated in Swedish Yorkshire pigs and F4 Wild Pig crosses. Levels of skatole, the skatole metabolite MIII, and androstenone were higher in males from the Wild Pig crosses than in the Yorkshire pigs. Plasma levels of the skatole metabolite MII and the ratio between MII and MIII were higher in female Yorkshire pigs than in male pigs. Skatole was correlated with androstenone, cytochrome P450IIE1, and estrone sulfate in the Wild Pig crosses but not in Yorkshire pigs. However, plasma levels of skatole metabolites were not correlated to levels of cytochrome P450IIE1 in liver in either group on animals. Pigs with high levels of cytochrome P450IIE1 in liver had low levels of skatole in fat, and pigs with low levels of cytochrome P450IIE1 in liver had low and high skatole levels. Using stepwise regression analysis, only levels of cytochrome P450IIE1 in liver significantly explained the variation in skatole levels in the Wild Pig crosses. Our results support the hypothesis that low levels of cytochrome P450IIE1 in liver may result in high levels of skatole in backfat of uncastrated male pigs due to decreased metabolism and clearance of skatole. However, these pigs cannot be identified based on plasma levels of skatole metabolites MII and MIII.
在瑞典约克夏猪和F4野猪杂交后代中，研究了脂肪中粪臭素和雄烯酮水平、粪臭素代谢物的血浆水平、血浆睾酮和硫酸雌酮以及肝脏中细胞色素P450IIE1水平之间的关系。野猪杂交后代的雄性猪中粪臭素、粪臭素代谢物MIII和雄烯酮的水平高于约克夏猪。雌性约克夏猪的血浆中粪臭素代谢物MII的水平以及MII与MIII的比值高于雄性猪。在野猪杂交后代中，粪臭素与雄烯酮、细胞色素P450IIE1和硫酸雌酮相关，但在约克夏猪中不相关。然而，两组动物中，血浆中粪臭素代谢物的水平与肝脏中细胞色素P450IIE1的水平均无相关性。肝脏中细胞色素P450IIE1水平高的猪，其脂肪中的粪臭素水平低；而肝脏中细胞色素P450IIE1水平低的猪，其脂肪中的粪臭素水平有高有低。通过逐步回归分析，只有肝脏中细胞色素P450IIE1的水平能显著解释野猪杂交后代中粪臭素水平的变化。我们的结果支持以下假设：由于粪臭素的代谢和清除减少，肝脏中细胞色素P450IIE1水平低可能导致未阉割雄性猪的背膘中粪臭素水平升高。然而，无法根据血浆中粪臭素代谢物MII和MIII的水平来识别这些猪。
