Brunius C, Vidanarachchi J K, Tomankova J, Lundström K, Andersson K, Zamaratskaia G
1Department of Food Science,Swedish University of Agricultural Sciences,BioCenter,PO Box 7051, SE-750 07 Uppsala,Sweden.
3Department of Animal Nutrition and Management,Swedish University of Agricultural Sciences,PO Box 7024, SE-750 07 Uppsala,Sweden.
Animal. 2016 Oct;10(10):1734-40. doi: 10.1017/S1751731116000574. Epub 2016 Apr 15.
Boar taint is a quality defect in meat, related to accumulation of skatole and androstenone in male pigs. The levels of skatole and its main metabolites in plasma and urine samples were measured with a validated liquid chromatography-MS method and related to activity of hepatic cytochrome P450 (CYP450) in order to identify 'fast metabolizing' pigs. Urine (n=46), blood (n=12), liver (n=25) and adipose tissue (n=46) were sampled from a total of 46 entire male pigs. Skatole levels in fat were negatively correlated to CYP2E1 activity and positively to 3-hydroxy-3-methyloxindole (HMOI), indole-3-carboxylic acid (ICA) and 2-aminoacetophenone in urine. HMOI and ICA levels in urine were the best predictors of high skatole levels in fat. In summary, the present study provided further evidence for the key role of CYP2E1 in skatole metabolism and suggested that measurement of HMOI and/or ICA in urine might provide information about skatole levels in live pigs.
公猪异味是猪肉中的一种品质缺陷，与雄性猪体内粪臭素和雄烯酮的积累有关。采用经过验证的液相色谱 - 质谱法测定血浆和尿液样本中粪臭素及其主要代谢物的水平，并将其与肝细胞色素P450（CYP450）的活性相关联，以识别“快速代谢”的猪。从总共46头完整雄性猪中采集尿液（n = 46）、血液（n = 12）、肝脏（n = 25）和脂肪组织（n = 46）。脂肪中粪臭素水平与CYP2E1活性呈负相关，与尿液中的3 - 羟基 - 3 - 甲基氧化吲哚（HMOI）、吲哚 - 3 - 羧酸（ICA）和2 - 氨基苯乙酮呈正相关。尿液中的HMOI和ICA水平是脂肪中高粪臭素水平的最佳预测指标。总之，本研究为CYP2E1在粪臭素代谢中的关键作用提供了进一步证据，并表明检测尿液中的HMOI和/或ICA可能提供有关生猪体内粪臭素水平的信息。
