Babol J, Squires E J, Lundström K
Department of Food Science, Swedish University of Agricultural Sciences, Uppsala.
J Anim Sci. 1998 Mar;76(3):829-38. doi: 10.2527/1998.763829x.
High concentrations of skatole in fat of some intact male pigs are a major cause of boar taint. In this study, we investigated the effect of oxidative and conjugative metabolism of skatole in liver on the concentrations of skatole in the fat of intact male pigs. In Trial 1, 18 Yorkshire intact males were equally divided into two treatments with high (mean, .42; SD, .26 ppm) and low (mean, .06; SD, .02 ppm) fat skatole levels. There was an increased rate of skatole metabolism, an increased glucuronidation activity, and a decreased sulfation activity toward 2-naphthol in liver from pigs with high skatole levels (P < .05). In Trial 2, Swedish Yorkshire x F4 European Wild Pig intact males were used. Among skatole metabolites that were produced in incubations with liver microsomes, pro-MII was conjugated with glucuronic acid and sulfate, and metabolite F-1 was conjugated with glucuronic acid. The rates of formation of various skatole metabolites and conjugation of pro-MII were evaluated for 22 pigs with different levels of cytochrome P4502E1 in the liver. The formation of F-1 and sulfation of pro-MII were negatively correlated with fat skatole levels (r = -.59, and r = -.56, respectively) and were decreased in pigs with high fat skatole levels and low P4502E1 levels (P < .01). The results indicate that oxidation and conjugation reactions of skatole in pig liver have a dramatic effect on skatole levels in the fat. In particular, the formation of F-1 and formation and subsequent sulfation of pro-MII are related to low levels of skatole in the fat, presumably due to rapid metabolic clearance of skatole.
一些未阉割雄性猪脂肪中高浓度的粪臭素是公猪异味的主要原因。在本研究中，我们调查了肝脏中粪臭素的氧化和结合代谢对未阉割雄性猪脂肪中粪臭素浓度的影响。在试验1中，18头约克夏未阉割雄性猪被平均分为两组，脂肪中粪臭素水平分别为高（平均0.42；标准差0.26 ppm）和低（平均0.06；标准差0.02 ppm）。粪臭素水平高的猪肝脏中，粪臭素代谢速率增加，葡萄糖醛酸化活性增加，对2-萘酚的硫酸化活性降低（P < 0.05）。在试验2中，使用了瑞典约克夏×F4欧洲野猪未阉割雄性猪。在与肝脏微粒体孵育产生的粪臭素代谢产物中，前MII与葡萄糖醛酸和硫酸盐结合，代谢产物F-1与葡萄糖醛酸结合。对22头肝脏中细胞色素P4502E1水平不同的猪，评估了各种粪臭素代谢产物的形成速率和前MII的结合情况。F-1的形成和前MII的硫酸化与脂肪中粪臭素水平呈负相关（分别为r = -0.59和r = -0.56），在脂肪中粪臭素水平高且P4502E1水平低的猪中降低（P < 0.01）。结果表明，猪肝中粪臭素的氧化和结合反应对脂肪中粪臭素水平有显著影响。特别是，F-1的形成以及前MII的形成和随后的硫酸化与脂肪中粪臭素水平低有关，推测是由于粪臭素的快速代谢清除。
