Domaratskaia E I, Starostin V I, Prianishnikova O D
Izv Akad Nauk Ser Biol. 1997 May-Jun(3):261-5.
The stem cell compartment of hemopoietic clonogenic cells (CFU-S) of mouse embryos in a closed system was studied, when the hemopoietic tissue was devoid of supply of the hemopoietic cells and grafted into adult recipients with diverse regulatory influences. The content of hemopoietic clonogenic cells (CFU-S-7, CFU-S-11, and CFU-S-el) was determined in the transplants of the 14-day embryonic liver placed under the renal capsule of preirradiated syngeneic adult recipients. Relatively younger clonogenic cells (CFU-S-11) were practically absent from the transplant in the end of cultivation, while the more advanced stages (CFU-S-7) became more abundant. CFU-S-el were preserved in the transplant for 24 h, and they were absent by the fourth day of cultivation. Disappearance of the clonogenic cells, which are referred to according to some properties as pre-CFU-S (CFU-S-el) from the stem compartment, a decreased content of another category of the clonogenic cells (CFU-S-11), and an increased content of the more advanced cells (CFU-S-7) suggest that in a closed system, maturation of the hemopoietic cells is accompanied by the exit of CFU-S to differentiation and is not compensated by their reproduction from the cells of earlier stages or self-reproduction.
在封闭系统中，研究了小鼠胚胎造血克隆形成细胞（CFU-S）的干细胞区室，此时造血组织缺乏造血细胞供应，并将其移植到受到不同调节影响的成年受体中。在置于经预照射的同基因成年受体肾被膜下的14天胚胎肝脏移植物中，测定了造血克隆形成细胞（CFU-S-7、CFU-S-11和CFU-S-el）的含量。在培养末期，移植物中几乎不存在相对较年轻的克隆形成细胞（CFU-S-11），而较晚期的细胞（CFU-S-7）则变得更加丰富。CFU-S-el在移植物中保存24小时，到培养第四天时消失。根据某些特性被称为前CFU-S（CFU-S-el）的克隆形成细胞从干细胞区室消失，另一类克隆形成细胞（CFU-S-11）的含量减少，而较晚期细胞（CFU-S-7）的含量增加，这表明在封闭系统中，造血细胞的成熟伴随着CFU-S向分化的转变，并且不能通过早期阶段细胞的繁殖或自我繁殖来补偿。
