Kajikawa H, Umemoto M, Taira E, Miki N, Mishiro Y, Kubo T, Yoneda Y
Department of Otolaryngology, Osaka University Medical School, Japan.
J Neurocytol. 1997 Jul;26(7):501-9. doi: 10.1023/a:1018585508713.
Several cell adhesion molecules are expressed in the developing inner ear. The present study focused on gicerin, a novel member of the immunoglobulin superfamily, in an attempt to improve our understanding of the development and regeneration of chick inner ear. Gicerin is known to homophilically interact with itself and to bind to neurite outgrowth factor (NOF). The data collected herein show that gicerin is highly expressed in auditory epithelium and acoustic ganglion during early embryogenesis. The immunoreactivity of gicerin in the auditory epithelium decreases more rapidly than that in the acoustic ganglion as the mature hair cells become distinguishable. At the post-hatch stage, the expression of gicerin is not observed. In contrast, NOF was expressed on the basement membranes around the auditory epithelium, and in the acoustic ganglion during development and after birth, but not in the auditory epithelium. Following noise damage, gicerin is transiently re-expressed on the damage receptor epithelium when active cell proliferation is observed in the epithelium. This positive reaction immediately disappears as immature short hair cells appear. These results suggest that gicerin may be associated with cell proliferation in the auditory epithelium, and play a role in neurite extension of the acoustic ganglion cells in conjunction with NOF.
几种细胞黏附分子在发育中的内耳中表达。本研究聚焦于免疫球蛋白超家族的新成员gicerin，旨在增进我们对鸡内耳发育和再生的理解。已知gicerin能与其自身进行同嗜性相互作用，并与神经突生长因子（NOF）结合。本文收集的数据表明，gicerin在胚胎发育早期的听觉上皮和听神经节中高表达。随着成熟毛细胞变得可分辨，听觉上皮中gicerin的免疫反应性比听神经节中的下降得更快。在孵化后阶段，未观察到gicerin的表达。相反，NOF在发育期间和出生后在听觉上皮周围的基底膜以及听神经节中表达，但在听觉上皮中不表达。噪声损伤后，当在上皮中观察到活跃的细胞增殖时，gicerin会在损伤的感受器上皮上短暂重新表达。当未成熟的短毛细胞出现时，这种阳性反应立即消失。这些结果表明，gicerin可能与听觉上皮中的细胞增殖有关，并与NOF一起在听神经节细胞的神经突延伸中发挥作用。
