Maruno M, Kovach J S, Kelly P J, Yanagihara T
Department of Neurology, Mayo Clinic, Rochester, Minnesota, USA.
J Clin Pathol. 1997 Jul;50(7):559-62. doi: 10.1136/jcp.50.7.559.
To determine the distribution and cellular origin of endogenous tumour necrosis factor alpha (TNF alpha) in the cellular components of human gliomas.
Frozen sections of 26 gliomas (four astrocytomas (As); two oligoastrocytomas (OA); one ansplastic astrocytoma (AA); one anaplastic oligoastrocytoma (AOA); 18 glioblastomas (GB)) were examined immunohistochemically using antihuman TNF alpha and anti-Leu-M5 (CD11c) antibodies. Additional studies with double immunohistocchemical procedures were performed with anti-glial fibrillary acidic protein and anti-neurofilament antibodies.
Eighty per cent of the AA, AOA, and GB (16 of 20) had a positive reaction for TNF alpha, but only 17% of As and OA (one of six) were positive. Positive cells were seen in both the tumour tissue and adjacent brain tissues. TNF alpha protein was detected not only in the tumour cells but also in the endothelium of tumour vessels as well as reactive astrocytes and neurons.
Endogenous TNF alpha is present in cells of various origins in glial tumours including tumour vessels; however, the role of TNF alpha may be different in different types of cells or altered microenvironment.
确定内源性肿瘤坏死因子α（TNFα）在人类胶质瘤细胞成分中的分布及细胞来源。
使用抗人TNFα和抗Leu-M5（CD11c）抗体，对26例胶质瘤（4例星形细胞瘤（As）；2例少突星形细胞瘤（OA）；1例间变性星形细胞瘤（AA）；1例间变性少突星形细胞瘤（AOA）；18例胶质母细胞瘤（GB））的冰冻切片进行免疫组织化学检查。另外采用抗胶质纤维酸性蛋白和抗神经丝抗体进行双重免疫组织化学程序的研究。
80%的AA、AOA和GB（20例中的16例）对TNFα呈阳性反应，但只有17%的As和OA（6例中的1例）呈阳性。在肿瘤组织和邻近脑组织中均可见阳性细胞。不仅在肿瘤细胞中检测到TNFα蛋白，在肿瘤血管内皮以及反应性星形胶质细胞和神经元中也检测到。
内源性TNFα存在于包括肿瘤血管在内的胶质肿瘤中各种来源的细胞中；然而，TNFα在不同类型细胞或改变的微环境中的作用可能不同。
