Forsblom B, Love D N, Sarkiala-Kessel E, Jousimies-Somer H
Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland.
Clin Infect Dis. 1997 Sep;25 Suppl 2:S100-6. doi: 10.1086/516209.
We characterized 52 anaerobic, gram-negative, nonpigmented, saccharolytic rods that were isolated from healthy and diseased subgingival sites of 16 family-owned dogs with spontaneous, clinically diagnosed periodontitis. Phenotypic features were determined with use of standard biochemical methods, by enzymatic profiling with the API ZYM system, and by cellular fatty acid profiling. Genotypic characterization was performed by DNA-DNA hybridization. Four phenotypic groups, defined as the Bacteroides fragilis group, the Prevotella buccae-like rods, the Prevotella heparinolytica/Prevotella zoogleoformans-like rods, and the slimy bile-tolerant rods, designated the Bacteroides pyogenes/Bacteroides tectum group, were detected. P. buccae and the B. pyogenes/B. tectum group organisms were isolated significantly more often (P values, < .05 and < .005) from the diseased than the healthy subgingival sites. The phenotypically similar group of bile-tolerant organisms, including B. pyogenes and B. tectum, most likely constitutes a major component of the anaerobic, gram-negative, saccharolytic microflora in periodontal lesions in dogs, a flora different from that in humans.
我们对从16只患有自发性、临床诊断为牙周炎的家养犬的健康和患病龈下部位分离出的52株厌氧、革兰氏阴性、无色素、解糖型杆菌进行了特性分析。通过使用标准生化方法、利用API ZYM系统进行酶谱分析以及进行细胞脂肪酸谱分析来确定表型特征。通过DNA-DNA杂交进行基因型鉴定。检测到四个表型组，分别定义为脆弱拟杆菌组、颊普雷沃菌样杆菌、解肝素普雷沃菌/菌胶团普雷沃菌样杆菌以及被指定为化脓性拟杆菌/隐蔽拟杆菌组的黏液耐胆盐杆菌。与健康龈下部位相比，颊普雷沃菌以及化脓性拟杆菌/隐蔽拟杆菌组的生物体在患病龈下部位的分离频率显著更高（P值分别<0.05和<0.005）。包括化脓性拟杆菌和隐蔽拟杆菌在内的表型相似的耐胆盐生物体组很可能构成犬牙周病变中厌氧、革兰氏阴性、解糖型微生物群落的主要成分，这一微生物群落与人类的不同。
