Glass R H, Ericsson S A, Ericsson R J, Drouin M T, Marcoux L J, Sullivan H
Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California-San Francisco 94123-0132.
Fertil Steril. 1991 Oct;56(4):743-6. doi: 10.1016/s0015-0282(16)54609-3.
The objective of the study was to determine if reduction of the dye resazurin by semen could be correlated with the concentration of motile sperm.
After assessment of sperm count and motility, specimens were incubated for 1 hour with resazurin (25 micrograms/mL of semen) and visual color changes indicative of dye reduction noted.
Specimens were obtained from men seeking care for infertility at one of four sites: (1) University of California, San Francisco (UCSF) In Vitro Fertilization Program; (2) UCSF Andrology Laboratory; (3) a gynecological practice in Maine; and (4) a private andrology laboratory in Southern California.
Individuals were self-selected by their desire to have a semen analysis in conjunction with the diagnosis or treatment of infertility.
None.
The reduction of the dye resazurin and its correlation with motile sperm density.
When the motile sperm concentration was greater than or equal to 20 X 10(6)/mL, 86% of specimens produced a positive color change. Conversely, 86% of specimens with a motile sperm concentration of less than 20 X 10(6)/mL either did not change color or changed only over a narrow range.
Reduction of resazurin offers an assessment of the active sperm in a specimen without the need to do a sperm count or evaluation of motility.
本研究的目的是确定精液对刃天青的还原作用是否与活动精子浓度相关。
在评估精子数量和活力后，将标本与刃天青（25微克/毫升精液）孵育1小时，并记录指示染料还原的视觉颜色变化。
标本取自四个地点之一寻求不育治疗的男性：（1）加利福尼亚大学旧金山分校（UCSF）体外受精项目；（2）UCSF男科实验室；（3）缅因州的一家妇科诊所；（4）南加州的一家私立男科实验室。
个体因希望结合不育诊断或治疗进行精液分析而自行选择。
无。
刃天青染料的还原及其与活动精子密度的相关性。
当活动精子浓度大于或等于20×10⁶/毫升时，86%的标本出现阳性颜色变化。相反，86%活动精子浓度低于20×10⁶/毫升的标本要么没有颜色变化，要么仅在狭窄范围内变化。
刃天青还原法可在无需进行精子计数或活力评估的情况下评估标本中的活性精子。
