O'Gorman E, Beutner G, Dolder M, Koretsky A P, Brdiczka D, Wallimann T
School of Biological Sciences, University of Manchester, UK.
FEBS Lett. 1997 Sep 8;414(2):253-7. doi: 10.1016/s0014-5793(97)01045-4.
Cyclosporin A sensitive swelling of mitochondria isolated from control mouse livers and from the livers of transgenic mice expressing human ubiquitous mitochondrial creatine kinase occurred in the presence of both 40 microM calcium and 5 microM atractyloside which was accompanied by a 2.5-fold increase over state 4 respiration rates. Creatine and cyclocreatine inhibited the latter only in transgenic liver mitochondria. Protein complexes isolated from detergent solubilised rat brain extracts, containing octameric mitochondrial creatine kinase, porin and the adenine nucleotide translocator, were reconstituted into malate loaded lipid vesicles. Dimerisation of creatine kinase in the complexes and exposure of the reconstituted complexes to >200 microM calcium induced a cyclosporin A sensitive malate release. No malate release occurred with complexes containing octameric creatine kinase under the same conditions.
从对照小鼠肝脏以及表达人普遍存在的线粒体肌酸激酶的转基因小鼠肝脏中分离出的线粒体，在40微摩尔钙和5微摩尔苍术苷存在的情况下会发生环孢素A敏感的肿胀，同时状态4呼吸速率增加了2.5倍。肌酸和环肌酸仅在转基因肝脏线粒体中抑制后者。从含有八聚体线粒体肌酸激酶、孔蛋白和腺嘌呤核苷酸转运体的去污剂溶解的大鼠脑提取物中分离出的蛋白质复合物，被重构成加载苹果酸的脂质体。复合物中肌酸激酶的二聚化以及重构复合物暴露于>200微摩尔钙会诱导环孢素A敏感的苹果酸释放。在相同条件下，含有八聚体肌酸激酶的复合物未发生苹果酸释放。
