Fell V, Lee C R
J Chromatogr. 1976 Jun 9;121(1):41-7. doi: 10.1016/s0021-9673(00)82296-7.
A procedure is described for the quantitative extraction of phenols from human urine. The compounds were chromatographed as the trimethylsilyl derivatives on wall-coated glass capillary columns. A more specific method of extraction involving the formation of acetate derivatives is also described. The acetates were prepared by treating dilute solutions of phenols at room temperature with acetic anhydride in the presence of 4-dimethylaminopyridine, which is a far more powerful catalyst than pyridine. Under these conditions N-acylamino acids are converted to azlactones.
本文描述了一种从人尿中定量提取酚类的方法。这些化合物以三甲基硅烷基衍生物的形式在涂壁玻璃毛细管柱上进行色谱分析。还描述了一种更具特异性的提取方法，该方法涉及形成乙酸酯衍生物。乙酸酯是通过在4-二甲基氨基吡啶存在下，于室温用乙酸酐处理酚类稀溶液而制备的，4-二甲基氨基吡啶是一种比吡啶更强效的催化剂。在这些条件下，N-酰基氨基酸会转化为恶唑烷酮。
