Ward G, McKinnon L, Badrick T, Hickman P E
Department of Chemical Pathology, Princess Alexandra Hospital, Woolloongabba, Queensland, Australia.
Am J Clin Pathol. 1997 Oct;108(4):417-21. doi: 10.1093/ajcp/108.4.417.
The prevalence of heterophilic antibody interference in a modern immunochemiluminometric assay containing blocking agents was determined using thyrotropin as an illustrative example. Serum samples were obtained from 295 consecutive patients who underwent routine thyroid function testing. The following versions of the thyrotropin assay were used: protocol A (zero blocker), protocol B (routine blocker concentration), and protocol C (extra blocker). Ten patients (prevalence 3.4%) had significant levels of heterophilic antibodies (protocol A value greater than 9 SD from the protocol B value). The observed thyrotropin levels for protocols B and C were the same for all patients, consistent with the reagent blockers in routine assays adequately eliminating heterophilic antibody interference. However, seven more patients (0.03%) in series of 21,000 assessed by routine thyroid function testing had discordant results because of a concentration of heterophilic antibodies so high as to overwhelm the added blocking agents.
以促甲状腺激素为例，测定了在含有封闭剂的现代免疫化学发光测定法中嗜异性抗体干扰的发生率。血清样本取自295例连续接受常规甲状腺功能检测的患者。使用了以下版本的促甲状腺激素测定法：方案A（零封闭剂）、方案B（常规封闭剂浓度）和方案C（额外封闭剂）。10名患者（发生率3.4%）的嗜异性抗体水平显著（方案A的值比方案B的值高9个标准差以上）。所有患者方案B和方案C的促甲状腺激素观察水平相同，这与常规检测中试剂封闭剂能充分消除嗜异性抗体干扰一致。然而，在21000例接受常规甲状腺功能检测的患者中，另有7名患者（0.03%）因嗜异性抗体浓度过高，超过了添加的封闭剂，结果不一致。
