Süsens U, Aguiluz J B, Evans R M, Borgmeyer U
Zentrum für Molekulare Neurobiologie, Universität Hamburg, Deutschland.
Dev Neurosci. 1997;19(5):410-20. doi: 10.1159/000111238.
Using a reinoic acid receptor hybridization probe, we have isolated a mouse embryonic cDNA that encodes the germ cell nuclear factor (mGCNF). The in vitro translated protein binds specifically to the direct repeat of the sequence-AGGTCA-which is characteristic of a subclass of nuclear receptors, albeit with a spacing of zero that is unique among the receptors. Northern analysis shows embryonic expression after mouse gastrulation and during early organogenesis, as well as expression in the adult testis. The message level decreases during embryonic development. Whereas there are two transcripts in the testis, only the larger one is found in embryos. In situ analysis of embryos at days 6.5-10.5 of gestation shows that, in the early postimplantation period, high transcript levels are found in ectodermal cells and in the primitive streak. During further development the expression becomes more restricted. Mainly cells of the developing nervous system are expressing the receptor. Our findings imply that GCNF is involved in two apparently disparate developmental events.
利用视黄酸受体杂交探针，我们分离出了一个小鼠胚胎cDNA，它编码生殖细胞核因子（mGCNF）。体外翻译的蛋白特异性结合序列-AGGTCA的直接重复序列，这是核受体亚类的特征，尽管其间隔为零，这在受体中是独特的。Northern分析显示，小鼠原肠胚形成后和早期器官发生期间有胚胎表达，以及在成年睾丸中也有表达。在胚胎发育过程中，信息水平下降。睾丸中有两种转录本，而在胚胎中只发现较大的一种。对妊娠6.5-10.5天的胚胎进行原位分析表明，在植入后早期，在外胚层细胞和原条中发现高转录水平。在进一步发育过程中，表达变得更加局限。主要是发育中的神经系统细胞在表达该受体。我们的发现表明，GCNF参与了两个明显不同的发育事件。
