Barchiesi F, Di Francesco L F, Compagnucci P, Arzeni D, Cirioni O, Scalise G
Istituto di Malattie Infettive & Medicina Pubblica, Università degli Studi di Ancona, Ospedale Umberto Io, Italy.
Eur J Clin Microbiol Infect Dis. 1997 Aug;16(8):601-5. doi: 10.1007/BF02447925.
Random amplification of polymorphic DNA and inter-repeat polymerase chain reaction (IR-PCR) were compared with restriction fragment length polymorphism (RFLP) analysis as methods for DNA typing of Candida albicans. Forty-seven strains of Candida albicans isolated from the oral cavities of five AIDS patients undergoing fluconazole therapy were analyzed. There was an excellent correspondence between the DNA types obtained by both PCR-based techniques and by RFLP. With the exception of one patient who was infected with three DNA types of Candida albicans during a five-year observation period, the patients each harboured only one major strain, which became progressively less susceptible to fluconazole. Each DNA type was unique to a patient. The data suggest that these typing methods are suitable for investigating the epidemiology of oropharyngeal candidiasis in this patient group.
将多态性DNA的随机扩增和重复序列间聚合酶链反应（IR-PCR）与限制性片段长度多态性（RFLP）分析进行比较，作为白色念珠菌DNA分型的方法。对从5名接受氟康唑治疗的艾滋病患者口腔中分离出的47株白色念珠菌进行了分析。基于PCR的技术和RFLP所获得的DNA类型之间存在极好的对应关系。在五年的观察期内，除了一名患者感染了三种白色念珠菌DNA类型外，其他患者各自仅携带一种主要菌株，该菌株对氟康唑的敏感性逐渐降低。每种DNA类型对于一名患者来说都是独特的。数据表明，这些分型方法适用于调查该患者群体中口腔念珠菌病的流行病学。
