van Belkum A, Melchers W, de Pauw B E, Scherer S, Quint W, Meis J F
Department of Molecular Biology, Diagnostic Center, Reinier de Graaf Gasthuis, Delft, Netherlands.
J Infect Dis. 1994 May;169(5):1062-70. doi: 10.1093/infdis/169.5.1062.
A polymerase chain reaction (PCR)-mediated genotyping assay for Candida albicans has been developed. By amplification of genomic regions bordered by eukaryotic or prokaryotic repeat-like motifs, differences between C. albicans isolates can be determined. The resolution of this typing procedure is at least as good as that of other genotypic assays. To ascertain the epidemiologic and clinical usefulness of this PCR genotyping, a retrospective analysis of serial C. albicans isolates from neutropenic adults treated with fluconazole was done. By PCR genotyping, 40 strains were detected in 24 patients. Eighteen C. albicans strains were found on multiple samplings in individual patients. It appears that most patients remain colonized with a C. albicans strain of constant genotypic characteristics. However, exceptions were observed. In 7 (29%) of 24 patients, strains deviating from the most frequently encountered type could be identified. All but 1 strain remained susceptible to fluconazole in vitro after treatment in vivo. It was not possible to demonstrate a relation of genotypic variation and antifungal susceptibility changes.
已开发出一种用于白色念珠菌的聚合酶链反应（PCR）介导的基因分型检测方法。通过扩增由真核或原核重复样基序界定的基因组区域，可以确定白色念珠菌分离株之间的差异。这种分型方法的分辨率至少与其他基因型检测方法一样好。为了确定这种PCR基因分型在流行病学和临床方面的实用性，对接受氟康唑治疗的中性粒细胞减少成人患者的系列白色念珠菌分离株进行了回顾性分析。通过PCR基因分型，在24例患者中检测到40株菌株。在个别患者的多次采样中发现了18株白色念珠菌菌株。看来大多数患者仍被具有恒定基因型特征的白色念珠菌菌株定植。然而，也观察到了例外情况。在24例患者中的7例（29%）中，可以鉴定出与最常见类型不同的菌株。除1株外，所有菌株在体内治疗后体外仍对氟康唑敏感。无法证明基因型变异与抗真菌药敏性变化之间的关系。
