棒状链霉菌头孢霉素簇的bla基因编码一种酶活性较低的A类β-内酰胺酶。
The bla gene of the cephamycin cluster of Streptomyces clavuligerus encodes a class A beta-lactamase of low enzymatic activity.
作者信息
Pérez-Llarena F, Martín J F, Galleni M, Coque J J, Fuente J L, Frère J M, Liras P
机构信息
Faculty of Biology, Department of Ecology, Genetics and Microbiology, University of Léon, Spain.
出版信息
J Bacteriol. 1997 Oct;179(19):6035-40. doi: 10.1128/jb.179.19.6035-6040.1997.
Abstract
A gene (bla) encoding a beta-lactamase is present in the cephamycin gene cluster of Streptomyces clavuligerus, the strain producing clavulanic acid and a beta-lactamase inhibitory protein. The bla gene is located 5.1 kb downstream from and in the opposite orientation to cefE, encoding the deacetoxycephalosporin C synthase. The bla gene encodes a 332-residue protein (Mr, 35,218), similar to other class A beta-lactamases produced by actinomycetes. Modification (to SDG) of the SDN conserved motif of class A beta-lactamases as well as of amino acids in otherwise conserved regions in the molecule may explain the low penicillinase and cephalosporinase activities of the protein. The beta-lactamase has been purified to homogeneity and found to bind [3H]benzylpenicillin, a result reflecting a rate-limiting deacylation step. Nucleotide sequences homologous to bla were found in all tested cephamycin producers, but several other Streptomyces species which produce a beta-lactamase do not contain genes for beta-lactam antibiotic biosynthesis.
摘要
编码β-内酰胺酶的基因（bla）存在于棒状链霉菌的头孢霉素基因簇中，该菌株可产生克拉维酸和一种β-内酰胺酶抑制蛋白。bla基因位于编码去乙酰氧头孢菌素C合酶的cefE下游5.1 kb处，且方向相反。bla基因编码一种由332个残基组成的蛋白质（Mr，35218），与放线菌产生的其他A类β-内酰胺酶相似。A类β-内酰胺酶的SDN保守基序以及分子中其他保守区域的氨基酸发生修饰（变为SDG），可能解释了该蛋白质青霉素酶和头孢菌素酶活性较低的原因。该β-内酰胺酶已被纯化至同质，并发现它能结合[3H]苄青霉素，这一结果反映了一个限速脱酰步骤。在所有测试的头孢霉素产生菌中都发现了与bla同源的核苷酸序列，但其他几种产生β-内酰胺酶的链霉菌物种并不包含β-内酰胺抗生素生物合成的基因。
相似文献
1
The bla gene of the cephamycin cluster of Streptomyces clavuligerus encodes a class A beta-lactamase of low enzymatic activity.棒状链霉菌头孢霉素簇的bla基因编码一种酶活性较低的A类β-内酰胺酶。
J Bacteriol. 1997 Oct;179(19):6035-40. doi: 10.1128/jb.179.19.6035-6040.1997.
2
A regulatory gene (ccaR) required for cephamycin and clavulanic acid production in Streptomyces clavuligerus: amplification results in overproduction of both beta-lactam compounds.产黄青霉中头孢霉素和克拉维酸生产所需的调控基因（ccaR）：扩增导致两种β-内酰胺化合物的过量生产。
J Bacteriol. 1997 Mar;179(6):2053-9. doi: 10.1128/jb.179.6.2053-2059.1997.
3
Effect of amplification or targeted disruption of the beta-lactamase gene of Nocardia lactamdurans on cephamycin biosynthesis.内酰胺分枝杆菌β-内酰胺酶基因的扩增或靶向破坏对头孢霉素生物合成的影响。
Appl Microbiol Biotechnol. 1996 Jun;45(5):621-8. doi: 10.1007/s002530050739.
4
Penicillin-Binding Proteins, β-Lactamases, and β-Lactamase Inhibitors in β-Lactam-Producing Actinobacteria: Self-Resistance Mechanisms.产β-内酰胺抗生素放线菌中的青霉素结合蛋白、β-内酰胺酶和β-内酰胺酶抑制剂：自身耐药机制。
Int J Mol Sci. 2022 May 18;23(10):5662. doi: 10.3390/ijms23105662.
5
Construction and analysis of ss-lactamase-inhibitory protein (BLIP) non-producer mutants of Streptomyces clavuligerus.棒状链霉菌β-内酰胺酶抑制蛋白（BLIP）非产生突变体的构建与分析
Microbiology (Reading). 2001 Feb;147(Pt 2):325-335. doi: 10.1099/00221287-147-2-325.
6
Genes for beta-lactam antibiotic biosynthesis.β-内酰胺抗生素生物合成相关基因。
Antonie Van Leeuwenhoek. 1995;67(2):181-200. doi: 10.1007/BF00871213.
7
Characterization and expression in Streptomyces lividans of cefD and cefE genes from Nocardia lactamdurans: the organization of the cephamycin gene cluster differs from that in Streptomyces clavuligerus.来自耐乳糖诺卡氏菌的cefD和cefE基因在变铅青链霉菌中的特性及表达：头霉素基因簇的组织形式与棒状链霉菌不同。
Mol Gen Genet. 1993 Jan;236(2-3):453-8. doi: 10.1007/BF00277148.
8
Sequence of a gene encoding beta-lactamase from Streptomyces cellulosae.来自纤维素链霉菌的β-内酰胺酶编码基因的序列。
Gene. 1993 Feb 14;124(1):111-4. doi: 10.1016/0378-1119(93)90769-y.
9
Genes for a beta-lactamase, a penicillin-binding protein and a transmembrane protein are clustered with the cephamycin biosynthetic genes in Nocardia lactamdurans.在耐内酰胺诺卡氏菌中，β-内酰胺酶、青霉素结合蛋白和跨膜蛋白的基因与头霉素生物合成基因聚集在一起。
EMBO J. 1993 Feb;12(2):631-9. doi: 10.1002/j.1460-2075.1993.tb05696.x.
10
The ddcA gene from Streptomyces fradiae encodes an extracellular beta-lactamase with penicillinase and cephalosporinase activities.来自弗氏链霉菌的ddcA基因编码一种具有青霉素酶和头孢菌素酶活性的细胞外β-内酰胺酶。
J Biotechnol. 2001 Nov 30;84(2):127-32. doi: 10.1016/s0168-1656(00)00341-2.
引用本文的文献
1
Distribution and Evolutionary Trajectories of β-Lactamases in Vibrio: Genomic Insights from Carbenicillin-Hydrolyzing Class A β-Lactamases (CARB) in the Harveyi and Cholerae Clades.弧菌中β-内酰胺酶的分布与进化轨迹：来自哈维氏弧菌和霍乱弧菌群中水解羧苄青霉素的A类β-内酰胺酶（CARB）的基因组见解
Genome Biol Evol. 2025 Jul 3;17(7). doi: 10.1093/gbe/evaf128.
2
Impact of Extract on Biofilm Formation for ESBL-: An Model Study.提取物对产超广谱β-内酰胺酶（ESBL）菌株生物膜形成的影响：一项模型研究
Pharmaceuticals (Basel). 2023 Sep 29;16(10):1383. doi: 10.3390/ph16101383.
3
Penicillin-Binding Proteins, β-Lactamases, and β-Lactamase Inhibitors in β-Lactam-Producing Actinobacteria: Self-Resistance Mechanisms.产β-内酰胺抗生素放线菌中的青霉素结合蛋白、β-内酰胺酶和β-内酰胺酶抑制剂：自身耐药机制。
Int J Mol Sci. 2022 May 18;23(10):5662. doi: 10.3390/ijms23105662.
4
In vivo functional analysis of a class A β-lactamase-related protein essential for clavulanic acid biosynthesis in Streptomyces clavuligerus.在体内对一株棒状链霉菌β-内酰胺酶相关蛋白进行功能分析，该蛋白对克拉维酸生物合成至关重要。
PLoS One. 2019 Apr 23;14(4):e0215960. doi: 10.1371/journal.pone.0215960. eCollection 2019.
5
Self-resistance in Streptomyces, with Special Reference to β-Lactam Antibiotics.链霉菌的自身抗性，特别涉及β-内酰胺抗生素
Molecules. 2016 May 10;21(5):605. doi: 10.3390/molecules21050605.
6
A Structure-Based Classification of Class A β-Lactamases, a Broadly Diverse Family of Enzymes.A类β-内酰胺酶的基于结构的分类，这是一个广泛多样的酶家族。
Clin Microbiol Rev. 2016 Jan;29(1):29-57. doi: 10.1128/CMR.00019-15.
7
Controlling resistant bacteria with a novel class of β-lactamase inhibitor peptides: from rational design to in vivo analyses.用新型β-内酰胺酶抑制肽控制耐药菌：从合理设计到体内分析
Sci Rep. 2014 Aug 11;4:6015. doi: 10.1038/srep06015.
8
Molecular analysis of β-lactamase genes to understand their differential expression in strains of Yersinia enterocolitica biotype 1A.β-内酰胺酶基因的分子分析，以了解它们在小肠结肠炎耶尔森菌1A生物型菌株中的差异表达。
Sci Rep. 2014 Jun 12;4:5270. doi: 10.1038/srep05270.
9
Molecular characterization of oxidative stress-inducible LipD of Mycobacterium tuberculosis H37Rv.结核分枝杆菌 H37Rv 中氧化应激诱导的 LipD 的分子特征。
Curr Microbiol. 2014 Mar;68(3):387-96. doi: 10.1007/s00284-013-0486-3.
10
Crystal structure and activity studies of the Mycobacterium tuberculosis beta-lactamase reveal its critical role in resistance to beta-lactam antibiotics.结核分枝杆菌β-内酰胺酶的晶体结构与活性研究揭示了其在对β-内酰胺类抗生素耐药性中的关键作用。
Antimicrob Agents Chemother. 2006 Aug;50(8):2762-71. doi: 10.1128/AAC.00320-06.
本文引用的文献
1
Effect of amplification or targeted disruption of the beta-lactamase gene of Nocardia lactamdurans on cephamycin biosynthesis.内酰胺分枝杆菌β-内酰胺酶基因的扩增或靶向破坏对头孢霉素生物合成的影响。
Appl Microbiol Biotechnol. 1996 Jun;45(5):621-8. doi: 10.1007/s002530050739.
2
Organization and expression in Pseudomonas putida of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90.来自产内酰胺溶杆菌属YK90的头孢菌素生物合成相关基因簇在恶臭假单胞菌中的组织与表达
Appl Microbiol Biotechnol. 1996 May;45(4):490-501. doi: 10.1007/BF00578461.
3
Construction of thiostrepton-inducible, high-copy-number expression vectors for use in Streptomyces spp.用于链霉菌属的硫链丝菌素诱导型高拷贝数表达载体的构建
Gene. 1995 Dec 1;166(1):133-7. doi: 10.1016/0378-1119(95)00545-2.
4
Genes for a beta-lactamase, a penicillin-binding protein and a transmembrane protein are clustered with the cephamycin biosynthetic genes in Nocardia lactamdurans.在耐内酰胺诺卡氏菌中，β-内酰胺酶、青霉素结合蛋白和跨膜蛋白的基因与头霉素生物合成基因聚集在一起。
EMBO J. 1993 Feb;12(2):631-9. doi: 10.1002/j.1460-2075.1993.tb05696.x.
5
The biosynthetic genes for clavulanic acid and cephamycin production occur as a 'super-cluster' in three Streptomyces.棒酸和头霉素生物合成基因在三种链霉菌中以“超级簇”的形式存在。
FEMS Microbiol Lett. 1993 Jun 15;110(2):239-42. doi: 10.1111/j.1574-6968.1993.tb06326.x.
6
Cloning, nucleotide sequence and expression of a beta-lactamase gene from Streptomyces lavendulae.来自淡紫链霉菌的β-内酰胺酶基因的克隆、核苷酸序列及表达
FEMS Microbiol Lett. 1993 Sep 15;112(3):343-8. doi: 10.1111/j.1574-6968.1993.tb06473.x.
7
Structural and kinetic characterization of a beta-lactamase-inhibitor protein.一种β-内酰胺酶抑制蛋白的结构与动力学特性
Nature. 1994 Apr 14;368(6472):657-60. doi: 10.1038/368657a0.
8
TEM beta-lactamase mutants hydrolysing third-generation cephalosporins. A kinetic and molecular modelling analysis.水解第三代头孢菌素的 TEM β-内酰胺酶突变体。动力学与分子模型分析
J Mol Biol. 1994 Dec 16;244(5):625-39. doi: 10.1006/jmbi.1994.1756.
9
Possible involvement of the lysine epsilon-aminotransferase gene (lat) in the expression of the genes encoding ACV synthetase (pcbAB) and isopenicillin N synthase (pcbC) in Streptomyces clavuligerus.赖氨酸ε-氨基转移酶基因（lat）可能参与产黄青霉中编码ACV合成酶（pcbAB）和异青霉素N合成酶（pcbC）的基因的表达。
Microbiology (Reading). 1994 Dec;140 ( Pt 12):3367-77. doi: 10.1099/13500872-140-12-3367.
10
Beta-lactamases and bacterial resistance to antibiotics.β-内酰胺酶与细菌对抗生素的耐药性。
Mol Microbiol. 1995 May;16(3):385-95. doi: 10.1111/j.1365-2958.1995.tb02404.x.
Zotero 插件