Kitagawa Y, Okuhara E
J Immunol Methods. 1976 Mar;10(2-3):151-9. doi: 10.1016/0022-1759(76)90166-6.
Immunological methods for the production of antibodies to DNA in ascitic fluid of DDY, A/He, and C57B1/6 mice were investigated. Relatively large volumes of ascitic fluid containing antibodies for DNA in high concentration were obtainable after seven injections of denatured ssDNA-MBSA complexes emulsified with CFA into peritoneal cavities of DDY mice. The quantity of anti-ascitic fluid from 4 to 5 DDY mice was equivalent to that of antiserum from one rabbit. DDY mice accumulation of ascites is much greater than in other strains, and the ascitic fluid contains antibodies of higher titer regardless of sex. The antibody titers in ascitic fluid are not affected by alteration of the ascites volume, and are proportional to those of the corresponding sera. Antibodies to DNA in ascitic fluid of these mice occurred exclusively in IgG as in DNA antisera.
研究了在DDY、A/He和C57B1/6小鼠腹水中产生抗DNA抗体的免疫学方法。在用CFA乳化的变性单链DNA-MBSA复合物七次注射到DDY小鼠腹腔后，可获得相对大量的含有高浓度抗DNA抗体的腹水。4至5只DDY小鼠的抗腹水产量相当于一只兔子的抗血清产量。DDY小鼠的腹水积聚比其他品系多得多，且无论性别，腹水中都含有更高滴度的抗体。腹水中的抗体滴度不受腹水量变化的影响，且与相应血清的滴度成正比。这些小鼠腹水中的抗DNA抗体仅以IgG形式出现，与DNA抗血清中的情况相同。
