Zirker D, Krueger G G, Meikle A W
J Invest Dermatol. 1976 Jun;66(6):376-8. doi: 10.1111/1523-1747.ep12482992.
Precutaneous absorption of dexamethasone and its effect on the pituitary adrenal axis were measured in vivo in normal human subjects after application to skin. Specific plasma dexamethasone and cortisol radioimmunoassays were used. Following application of 1% dexamethasone on 500 cm2 of normal skin, the plasma dexamethasone concentration was maximal at 2 hr, and the average absorption was 0.25% over 8 hr; significant cortisol suppression occurred at 2, 4, and 8 hr. This technique: (1) provides an accurate assessment of the in vivo absorption of dexamethasone applied to human skin, (2) avoids exposure of the subjects to radioactive steroids, (3)permits estimation of the quantity of unmetabolized steroids absorbed, and (4) serves as a possible model for the development of similar assays for other topical steroids.
在正常人体受试者的皮肤上涂抹地塞米松后，对其经皮吸收情况及其对垂体-肾上腺轴的影响进行了体内测量。使用了特异性血浆地塞米松和皮质醇放射免疫测定法。在500平方厘米的正常皮肤上涂抹1%地塞米松后，血浆地塞米松浓度在2小时时达到最高，8小时内的平均吸收率为0.25%；在2小时、4小时和8小时时出现了显著的皮质醇抑制。该技术：（1）可准确评估涂抹于人体皮肤的地塞米松的体内吸收情况，（2）避免受试者接触放射性类固醇，（3）能够估算吸收的未代谢类固醇的量，（4）可作为开发其他局部用类固醇类似检测方法的潜在模型。
