Farias R N, Torres M, Canela R
Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (CONICET-UNT), San Miguel de Tucumán, Argentina.
Anal Biochem. 1997 Oct 1;252(1):186-9. doi: 10.1006/abio.1997.2240.
Using commercially available thiosubstrates, such as 2,3-dimercapto-1-propanol tributyrate, the regio-specificities of 1,3-specific and nonspecific lipases was confirmed. The spectrophotometric test is a simple, rapid, and convenient alternative method to those previously reported for the characterization of the positional specificities of new lipases.
使用市售硫代底物，如2,3-二巯基-1-丙醇三丁酸酯，证实了1,3-特异性和非特异性脂肪酶的区域特异性。分光光度法测试是一种简单、快速且方便的替代方法，可用于表征新型脂肪酶的位置特异性，相较于之前报道的方法。
