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从潮滩沉积物宏基因组文库中分离并鉴定一种新型脂肪酶：一个新的细菌脂肪酶家族的证据

Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacterial lipases.

作者信息

Lee Mi-Hwa, Lee Choong-Hwan, Oh Tae-Kwang, Song Jae Kwang, Yoon Jung-Hoon

机构信息

Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.

出版信息

Appl Environ Microbiol. 2006 Nov;72(11):7406-9. doi: 10.1128/AEM.01157-06. Epub 2006 Sep 1.

DOI:10.1128/AEM.01157-06
PMID:16950897
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1636159/
Abstract

We cloned lipG, which encoded a lipolytic enzyme, from a Korean tidal flat metagenomic library. LipG was related to six putative lipases previously identified only in bacterial genome sequences. These enzymes comprise a new family. We partially characterized LipG, providing the first experimental data for a member of this family.

摘要

我们从一个韩国潮间带宏基因组文库中克隆了编码脂肪分解酶的lipG。LipG与之前仅在细菌基因组序列中鉴定出的六种假定脂肪酶相关。这些酶构成了一个新家族。我们对LipG进行了部分表征，为该家族的一个成员提供了首个实验数据。

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Appl Microbiol Biotechnol. 2006 Apr;70(3):321-6. doi: 10.1007/s00253-005-0058-y. Epub 2005 Aug 9.
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Isolation of novel lipolytic genes from uncultured bacteria of pond water.从池塘水未培养细菌中分离新型脂肪分解基因。
Biochem Biophys Res Commun. 2005 Sep 16;335(1):57-65. doi: 10.1016/j.bbrc.2005.07.046.
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The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses.通过基因组和蛋白质组分析，嗜冷红杆菌34H的基因组序列所揭示的嗜冷生活方式。
Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):10913-8. doi: 10.1073/pnas.0504766102. Epub 2005 Jul 25.
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Metagenomics and industrial applications.宏基因组学与工业应用。
Nat Rev Microbiol. 2005 Jun;3(6):510-6. doi: 10.1038/nrmicro1161.
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The metagenomics of soil.土壤宏基因组学
Nat Rev Microbiol. 2005 Jun;3(6):470-8. doi: 10.1038/nrmicro1160.
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Archaeal diversity in tidal flat sediment as revealed by 16S rDNA analysis.基于16S rDNA分析揭示的潮滩沉积物中古菌多样性
J Microbiol. 2005 Apr;43(2):144-51.
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New thermophilic and thermostable esterase with sequence similarity to the hormone-sensitive lipase family, cloned from a metagenomic library.从宏基因组文库中克隆出的与激素敏感脂肪酶家族具有序列相似性的新型嗜热和耐热酯酶。
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Metagenomics--the key to the uncultured microbes.宏基因组学——未培养微生物的关键
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