从潮滩沉积物宏基因组文库中分离并鉴定一种新型脂肪酶：一个新的细菌脂肪酶家族的证据
Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacterial lipases.
作者信息
Lee Mi-Hwa, Lee Choong-Hwan, Oh Tae-Kwang, Song Jae Kwang, Yoon Jung-Hoon
机构信息
Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
出版信息
Appl Environ Microbiol. 2006 Nov;72(11):7406-9. doi: 10.1128/AEM.01157-06. Epub 2006 Sep 1.
Abstract
We cloned lipG, which encoded a lipolytic enzyme, from a Korean tidal flat metagenomic library. LipG was related to six putative lipases previously identified only in bacterial genome sequences. These enzymes comprise a new family. We partially characterized LipG, providing the first experimental data for a member of this family.
摘要
我们从一个韩国潮间带宏基因组文库中克隆了编码脂肪分解酶的lipG。LipG与之前仅在细菌基因组序列中鉴定出的六种假定脂肪酶相关。这些酶构成了一个新家族。我们对LipG进行了部分表征，为该家族的一个成员提供了首个实验数据。
相似文献
1
Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacterial lipases.
Appl Environ Microbiol. 2006 Nov;72(11):7406-9. doi: 10.1128/AEM.01157-06. Epub 2006 Sep 1.
2
Molecular cloning and characterization of a new cold-active esterase from a deep-sea metagenomic library.
Appl Microbiol Biotechnol. 2011 May;90(3):961-70. doi: 10.1007/s00253-010-3079-0. Epub 2011 Feb 19.
3
Isolation and characterization of a new alkali-thermostable lipase cloned from a metagenomic library.
J Ind Microbiol Biotechnol. 2009 Jun;36(6):853-61. doi: 10.1007/s10295-009-0562-7. Epub 2009 Mar 31.
4
Identification and characterization of novel esterases from a deep-sea sediment metagenome.
Arch Microbiol. 2012 Mar;194(3):207-14. doi: 10.1007/s00203-011-0745-2. Epub 2011 Aug 23.
5
Metagenomic approach for the isolation of a novel low-temperature-active lipase from uncultured bacteria of marine sediment.
FEMS Microbiol Ecol. 2007 Feb;59(2):524-34. doi: 10.1111/j.1574-6941.2006.00206.x.
6
Isolation of a novel lipase from a metagenomic library derived from mangrove sediment from the south Brazilian coast.
Genet Mol Res. 2010 Mar 23;9(1):514-23. doi: 10.4238/vol9-1gmr738.
7
Novel metagenome-derived, cold-adapted alkaline phospholipase with superior lipase activity as an intermediate between phospholipase and lipase.
Appl Environ Microbiol. 2012 Jul;78(14):4959-66. doi: 10.1128/AEM.00260-12. Epub 2012 Apr 27.
8
Novel lipolytic genes from the microbial metagenomic library of the South China Sea marine sediment.
FEMS Microbiol Ecol. 2010 May;72(2):228-37. doi: 10.1111/j.1574-6941.2010.00851.x. Epub 2010 Feb 16.
9
Isolation and biochemical characterization of two lipases from a metagenomic library of China Holstein cow rumen.
Biochem Biophys Res Commun. 2009 Aug 7;385(4):605-11. doi: 10.1016/j.bbrc.2009.05.110. Epub 2009 May 30.
10
A novel esterase gene cloned from a metagenomic library from neritic sediments of the South China Sea.
Microb Cell Fact. 2011 Nov 9;10:95. doi: 10.1186/1475-2859-10-95.
引用本文的文献
1
Molecular Cloning and Characterization of a New Family VI Esterase from an Activated Sludge Metagenome.
Microorganisms. 2022 Dec 4;10(12):2403. doi: 10.3390/microorganisms10122403.
2
Characterization of a Novel Moderately Thermophilic Solvent-Tolerant Esterase Isolated From a Compost Metagenome Library.
Front Microbiol. 2020 Jan 24;10:3069. doi: 10.3389/fmicb.2019.03069. eCollection 2019.
3
A proposed update for the classification and description of bacterial lipolytic enzymes.
PeerJ. 2019 Jul 8;7:e7249. doi: 10.7717/peerj.7249. eCollection 2019.
4
Characterization of ML-005, a Novel Metaproteomics-Derived Esterase.
Front Microbiol. 2018 Aug 22;9:1925. doi: 10.3389/fmicb.2018.01925. eCollection 2018.
5
Bacillus sp. JR3 esterase LipJ: A new mesophilic enzyme showing traces of a thermophilic past.
PLoS One. 2017 Jul 25;12(7):e0181029. doi: 10.1371/journal.pone.0181029. eCollection 2017.
6
Screening, purification, and characterization of a novel organic solvent-tolerant esterase, Lip2, from Monascus purpureus strain M7.
Extremophiles. 2017 Mar;21(2):345-355. doi: 10.1007/s00792-016-0907-x. Epub 2017 Jan 6.
7
Metagenomic mining for thermostable esterolytic enzymes uncovers a new family of bacterial esterases.
Sci Rep. 2016 Dec 19;6:38886. doi: 10.1038/srep38886.
8
Thermus thermophilus as a Source of Thermostable Lipolytic Enzymes.
Microorganisms. 2015 Nov 4;3(4):792-808. doi: 10.3390/microorganisms3040792.
9
Isolation and Characterization of a Glycosyl Hydrolase Family 16 β-Agarase from a Mangrove Soil Metagenomic Library.
Int J Mol Sci. 2016 Aug 19;17(8):1360. doi: 10.3390/ijms17081360.
10
Integrative computational approach for genome-based study of microbial lipid-degrading enzymes.
World J Microbiol Biotechnol. 2016 Jul;32(7):122. doi: 10.1007/s11274-016-2067-7. Epub 2016 Jun 4.
本文引用的文献
1
New cold-adapted lipase from Photobacterium lipolyticum sp. nov. that is closely related to filamentous fungal lipases.
Appl Microbiol Biotechnol. 2006 Apr;70(3):321-6. doi: 10.1007/s00253-005-0058-y. Epub 2005 Aug 9.
2
Isolation of novel lipolytic genes from uncultured bacteria of pond water.
Biochem Biophys Res Commun. 2005 Sep 16;335(1):57-65. doi: 10.1016/j.bbrc.2005.07.046.
3
The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses.
Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):10913-8. doi: 10.1073/pnas.0504766102. Epub 2005 Jul 25.
4
Metagenomics and industrial applications.
Nat Rev Microbiol. 2005 Jun;3(6):510-6. doi: 10.1038/nrmicro1161.
5
The metagenomics of soil.
Nat Rev Microbiol. 2005 Jun;3(6):470-8. doi: 10.1038/nrmicro1160.
6
Archaeal diversity in tidal flat sediment as revealed by 16S rDNA analysis.
J Microbiol. 2005 Apr;43(2):144-51.
7
New thermophilic and thermostable esterase with sequence similarity to the hormone-sensitive lipase family, cloned from a metagenomic library.
Appl Environ Microbiol. 2005 Feb;71(2):817-25. doi: 10.1128/AEM.71.2.817-825.2005.
8
Metagenomics--the key to the uncultured microbes.
Curr Opin Microbiol. 2004 Oct;7(5):492-8. doi: 10.1016/j.mib.2004.08.002.
9
Diverse catalytic activities in the alphabeta-hydrolase family of enzymes: activation of H2O, HCN, H2O2, and O2.
Bioorg Chem. 2004 Oct;32(5):367-75. doi: 10.1016/j.bioorg.2004.05.005.
10
Screening for novel lipolytic enzymes from uncultured soil microorganisms.
Appl Microbiol Biotechnol. 2004 Nov;65(6):720-6. doi: 10.1007/s00253-004-1722-3. Epub 2004 Sep 10.
Zotero 插件