Fathman C G, Hengartner H
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5863-6. doi: 10.1073/pnas.76.11.5863.
We have isolated clones of alloreactive T cells by soft agar cloning of primed responder cells contained in serially restimulated, mixed lymphocyte cultures. These clones recognize unique mixed lymphocyte reaction (MLR) stimulating determinants present on (C57B/6 X A/J)F1 (B6A) spleen cells. By assaying the reactivity patterns of these clones on a panel of stimulator cells, we have identified at least three separate "specificities" of alloreactive T cell clones. Several clones exhibiting one of these three reactivity patterns have been isolated on many occasions from different experiments. Using such clones of alloreactive T cells, we have identified and are attempting to define crossreactive MLR stimulating determinants exhibited by a panel of stimulator cells. Recognition by certain clones of a shared MLR determinant(s) on B6A and DBA/2 and of the lack of this determinant(s) on B6 stimulator cells questions current concepts concerning the nature of MLR stimulating determinants and T cell recognition of alloantigens.
我们通过对连续再刺激的混合淋巴细胞培养物中含有的致敏应答细胞进行软琼脂克隆,分离出了同种异体反应性T细胞克隆。这些克隆识别存在于(C57B/6×A/J)F1(B6A)脾细胞上的独特混合淋巴细胞反应(MLR)刺激决定簇。通过检测这些克隆对一组刺激细胞的反应模式,我们确定了同种异体反应性T细胞克隆至少三种不同的“特异性”。在许多不同的实验中,多次分离出表现出这三种反应模式之一的几个克隆。利用这些同种异体反应性T细胞克隆,我们已经鉴定并正在试图确定一组刺激细胞所呈现的交叉反应性MLR刺激决定簇。某些克隆对B6A和DBA/2上共同的MLR决定簇的识别以及对B6刺激细胞上缺乏该决定簇的识别,对当前有关MLR刺激决定簇的性质和T细胞对同种异体抗原识别的概念提出了质疑。