Lohrmann R, Orgel L E
J Mol Evol. 1976 May 26;7(4):253-67. doi: 10.1007/BF01743625.
2'-Amino-2'-deoxyuridine reacts efficiently with nucleoside 5'-phosphorimidazolides in aqueous solution. The dinucleoside monophosphate analogues were obtained in yields exceeding 80% under conditions in which little reaction occurs with the natural nucleosides. In a similar way, the 5'-phosphorimidazolide of 2'-amino-2'-deoxyuridine undergoes self-condensation in aqueous solution to give a complex mixture of oligomers. The phosphoramidate bond in the dinucleoside monophosphate analogues is stable for several days at room temperature and pH 7. The mechanisms of their hydrolysis under acidic and alkaline conditions are described.
2'-氨基-2'-脱氧尿苷在水溶液中能与核苷5'-磷酰咪唑发生高效反应。在与天然核苷几乎不发生反应的条件下，可获得产率超过80%的二核苷单磷酸类似物。同样，2'-氨基-2'-脱氧尿苷的5'-磷酰咪唑在水溶液中会发生自缩合反应，生成复杂的低聚物混合物。二核苷单磷酸类似物中的磷酰胺键在室温及pH 7条件下可稳定存在数天。文中描述了它们在酸性和碱性条件下的水解机制。
