Ivanova S A, Shekhonin B V, Chumakov A A
Arkh Patol. 1997 Jul-Aug;59(4):49-55.
Phenotypic characteristics of cells and extracellular matrix (ECM) of 14 salivary gland pleomorphic adenomas were studied by indirect immunofluorescence. All cells in the epithelial structures expressed cytokeratin 8, the majority of cells produced vimentin, laminin and IV type collagen. Cells of myxoid areas expressed vimentin: type IV collagen was observed in the form of fibrillar structures. Fibrous areas were characterized by vimentin and cytokeratin 8 expression; types I, III, IV collagens and fibronectin with ED-A sequence were present in ECM. Terms "epithelial structures", "epithelial" and "myoepithelial" cells in relation to pleomorphic adenoma may be used only relatively as it is mainly represented by cells with features of both epithelial and mesenchymal phenotype. Characteristics of ECM synthesized in various structures of pleomorphic adenoma may serve an additional criterion of cell transformation.
通过间接免疫荧光法研究了14例涎腺多形性腺瘤细胞和细胞外基质（ECM）的表型特征。上皮结构中的所有细胞均表达细胞角蛋白8，大多数细胞产生波形蛋白、层粘连蛋白和IV型胶原。黏液样区域的细胞表达波形蛋白；IV型胶原呈纤维状结构。纤维区域的特征是波形蛋白和细胞角蛋白8的表达；ECM中存在I型、III型、IV型胶原以及具有ED-A序列的纤连蛋白。与多形性腺瘤相关的术语“上皮结构”、“上皮”和“肌上皮”细胞只能相对使用，因为它主要由具有上皮和间充质表型特征的细胞组成。多形性腺瘤不同结构中合成的ECM特征可能是细胞转化的另一个标准。
