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2
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Stabilization of DNA triple-helix formation by appended cationic peptides.
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DNA condensation.DNA凝聚
Curr Opin Struct Biol. 1996 Jun;6(3):334-41. doi: 10.1016/s0959-440x(96)80052-2.
3
Oligonucleotide directed triple helix formation.寡核苷酸定向三链螺旋形成。
Curr Opin Struct Biol. 1996 Jun;6(3):327-33. doi: 10.1016/s0959-440x(96)80051-0.
4
Triplex formation at physiological pH by 5-Me-dC-N4-(spermine) [X] oligodeoxynucleotides: non protonation of N3 in X of X*G:C triad and effect of base mismatch/ionic strength on triplex stabilities.5-甲基脱氧胞苷-N4-（精胺）[X]寡脱氧核苷酸在生理pH下形成三链体：X*G:C三联体中X的N3不发生质子化以及碱基错配/离子强度对三链体稳定性的影响
Nucleic Acids Res. 1996 Apr 1;24(7):1229-37. doi: 10.1093/nar/24.7.1229.
5
7,8-Dihydro-8-oxoadenine as a replacement for cytosine in the third strand of triple helices. Triplex formation without hypochromicity.7,8-二氢-8-氧代腺嘌呤作为三链螺旋体第三条链中胞嘧啶的替代物。形成无减色效应的三链体。
Biochemistry. 1993 Apr 6;32(13):3249-54. doi: 10.1021/bi00064a006.
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Polyamine-linked oligonucleotides for DNA triple helix formation.用于形成DNA三螺旋的多胺连接寡核苷酸。
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Electrostatic effects in DNA triple helices.
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Triplex formation at physiological pH by oligonucleotides incorporating 5-Me-dC-(N4-spermine).在生理pH值下，由掺入5-甲基-dC-（N4-精胺）的寡核苷酸形成三链体。
Biochem Biophys Res Commun. 1994 Dec 30;205(3):1665-70. doi: 10.1006/bbrc.1994.2859.

生理pH条件下的三链体形成：含N4位连接5-甲基脱氧胞苷的DNA三链体与精胺和四乙烯氧基胺的比较研究

Triplex formation at physiological pH: comparative studies on DNA triplexes containing 5-Me-dC tethered at N4 with spermine and tetraethyleneoxyamine.

作者信息

Rajeev K G, Jadhav V R, Ganesh K N

机构信息

Division of Organic Chemistry, National Chemical Laboratory, Pune 411008, India.

出版信息

Nucleic Acids Res. 1997 Nov 1;25(21):4187-93. doi: 10.1093/nar/25.21.4187.

DOI:10.1093/nar/25.21.4187
PMID:9336445
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC147060/
Abstract

Oligodeoxynucleotides with spermine conjugation at C4 of 5-Me-dC ( sp -ODN) exhibit triple helix formation with complementary Watson-Crick duplexes, and were optimally stable at physiological pH 7.3 and low salt concentration. This was attributed to a favored reassociation of the polycationic third strand with the anionic DNA duplex. To gain further insights into the factors that contribute to the enhancement of triplex stability and for engineering improved triplex systems, the spermine appendage at C4 of 5-Me-dC was replaced with 1,11-diamino-3,6,9-trioxaundecane to create teg -ODNs. From the triple helix forming abilities of these modified ODNs studied by hysteresis behaviour and the effect of salts on triplex stability, it is demonstrated here that teg- ODNs stabilise triplexes through hydrophobic desolvation while sp -ODNs stabilise triplexes by charge effects. The results imply that factors in addition to base stacking effects and interstrand hydrogen bonds are significantly involved in modulation of triplex stability by base modified oligonucleotides.

摘要

在5-甲基脱氧胞苷（5-Me-dC）的C4位与精胺偶联的寡脱氧核苷酸（sp-ODN）可与互补的沃森-克里克双链体形成三链螺旋，并且在生理pH 7.3和低盐浓度下具有最佳稳定性。这归因于聚阳离子第三链与阴离子DNA双链体的有利重新结合。为了进一步深入了解有助于增强三链体稳定性的因素并构建改进的三链体系统，将5-Me-dC的C4位上的精胺附属物替换为1,11-二氨基-3,6,9-三氧杂十一烷以制备teg-ODN。通过滞后行为研究这些修饰的ODN的三链螺旋形成能力以及盐对三链体稳定性的影响，本文证明teg-ODN通过疏水去溶剂化作用稳定三链体，而sp-ODN通过电荷效应稳定三链体。结果表明，除了碱基堆积效应和链间氢键外，碱基修饰的寡核苷酸在调节三链体稳定性方面还涉及其他重要因素。