Yoon J H, Rhee S K, Lee J S, Park Y H, Lee S T
Korean Collection for Type Cultures, Korea Research Institute of Bioscience & Biotechnology, Yusong, Taejon, Korea.
Int J Syst Bacteriol. 1997 Oct;47(4):933-8. doi: 10.1099/00207713-47-4-933.
A bacterial strain which is able to degrade pyridine was previously isolated from the oxic zone of an oil shale column and described as Pimelobacter sp. strain OS4T. However, Pimelobacter species have been transferred to the genera Nocardioides and Terrabacter. Strain OS4T was identified as a member of the genus Nocardioides on the basis of chemotaxonomic analysis and phylogenetic inference based on 16S ribosomal DNA (rDNA) sequence analysis. The G+C content of strain OS4T is 72.5 mol%. The cell wall peptidoglycan contains LL-diaminopimelic acid as the diamino acid. The predominant menaquinone is MK-8(H4). The cellular fatty acid profile of strain OS4T is similar to that of the genus Nocardioides. The 16S rDNA similarity of strain OS4T with previously described Nocardioides species is 94.5% +/- 0.7%, and a phylogenetic tree based on 16S rDNA sequences revealed a distinct lineage for strain OS4T within the evolutionary radiation enclosed by the genus Nocardioides. Therefore, on the basis of our data, we propose that strain OS4T should be placed in the genus Nocardioides as a member of a new species, Nocardioides pyridinolyticus. The type strain of the new species is strain OS4 (= KCTC 0074BP).
一株能够降解吡啶的细菌菌株先前是从油页岩柱的好氧区分离出来的，并被描述为皮氏杆菌属菌株OS4T。然而，皮氏杆菌属已被转移到诺卡氏菌属和土壤杆菌属。基于化学分类分析以及基于16S核糖体DNA（rDNA）序列分析的系统发育推断，菌株OS4T被鉴定为诺卡氏菌属的一个成员。菌株OS4T的G+C含量为72.5摩尔%。细胞壁肽聚糖含有LL-二氨基庚二酸作为二氨基酸。主要的甲基萘醌是MK-8(H4)。菌株OS4T的细胞脂肪酸谱与诺卡氏菌属相似。菌株OS4T与先前描述的诺卡氏菌属物种的16S rDNA相似性为94.5%±0.7%，基于16S rDNA序列的系统发育树显示，在诺卡氏菌属所包含的进化辐射范围内，菌株OS4T有一个独特的谱系。因此，根据我们的数据，我们提议将菌株OS4T作为一个新物种——解吡啶诺卡氏菌的成员归入诺卡氏菌属。新物种的模式菌株是菌株OS4（=KCTC 0074BP）。
