Fernandez-Garayzabal J F, Collins M D, Hutson R A, Fernandez E, Monasterio R, Marco J, Dominguez L
Departamento Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Int J Syst Bacteriol. 1997 Oct;47(4):1082-5. doi: 10.1099/00207713-47-4-1082.
Fourteen strains of a hitherto unknown catalase-positive, aerobic, gram-positive coryneformlike organism were isolated from the milk of sheep with subclinical mastitis from different regions of Spain. The strains phenotypically closely resembled one another and biochemically were similar to Corynebacterium urealyticum and Corynebacterium afermentans subsp. lipophilum. The results of chemotaxonomic investigations were consistent with membership in the genus Corynebacterium, and comparative 16S rRNA gene sequencing studies showed that the unknown bacterium from sheep was indeed a member of the genus Corynebacterium. Within the genus Corynebacterium the new bacterium formed a distinct subline that exhibited > 4% sequence divergence with other species. Based on both phenotypic and phylogenetic findings, a new species, Corynebacterium mastitidis, is proposed for the organisms from mastitic sheep. The type strain of C. mastitidis is CECT 4843 (= S-8).
从西班牙不同地区患有亚临床乳腺炎的绵羊乳汁中分离出14株此前未知的过氧化氢酶阳性、需氧、革兰氏阳性棒状杆菌样微生物。这些菌株在表型上彼此非常相似，在生化特性上与解脲棒状杆菌和嗜脂发酵棒状杆菌亚种相似。化学分类学研究结果与棒状杆菌属成员一致，比较16S rRNA基因测序研究表明，来自绵羊的未知细菌确实是棒状杆菌属的一员。在棒状杆菌属内，这种新细菌形成了一个独特的亚系，与其他物种表现出>4%的序列差异。基于表型和系统发育研究结果，为来自患乳腺炎绵羊的这些微生物提出了一个新物种——乳房炎棒状杆菌。乳房炎棒状杆菌的模式菌株是CECT 4843（=S-8）。
