Psychiatry Clinic "Dr Selaković", Belgrade, Serbia.
Department of Biochemistry, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
Front Cell Infect Microbiol. 2020 May 19;10:223. doi: 10.3389/fcimb.2020.00223. eCollection 2020.
While gut microbiota dysbiosis has been linked with autism, its role in the etiology of other neurodevelopmental disorders (NDD) is largely underexplored. To our knowledge this is the first study to evaluate gut microbiota diversity and composition in 36 children from the Republic of Serbia diagnosed with NDD and 28 healthy children. The results revealed an increased incidence of potentially harmful bacteria, closely related to species, in the NDD patient group compared to the Control group: ( < 0.01), ( < 0.05), and ( < 0.001). On the other hand, significantly lower diversity of common commensal bacteria in the NDD group of patients was noticed. ( < 0.05), ( < 0.01), ( < 0.05), ( < 0.01) and sp. were detected in lower numbers of patients or were even absent in some NDD patients. In addition, butyrate-producing bacteria ( < 0.01), ( < 0.05), and ( = 0.07) were less frequent in the NDD patient group. In line with that, the levels of fecal short chain fatty acids (SCFAs) were determined. Although significant differences in SCFA levels were not detected between NDD patients and the Control group, a positive correlation was noted between number of rDNA amplicons obtained with universal primers and level of propionic acid, as well as a trend for levels of total SCFAs and butyric acid in the Control group. This correlation is lost in the NDD patient group, indicating that NDD patients' microbiota differs from the microbiota of healthy children in the presence or number of strong SCFA-producing bacteria. According to a range-weighted richness index it was observed that microbial diversity was significantly lower in the NDD patient group. Our study reveals that the intestinal microbiota from NDD patients differs from the microbiota of healthy children. It is hypothesized that early life microbiome might have an impact on GI disturbances and accompanied behavioral problems frequently observed in patients with a broad spectrum of NDD.
虽然肠道微生物群失调与自闭症有关，但它在其他神经发育障碍（NDD）病因学中的作用在很大程度上尚未得到充分探索。据我们所知，这是第一项评估塞尔维亚共和国 36 名 NDD 患儿和 28 名健康儿童肠道微生物多样性和组成的研究。结果显示，与对照组相比，NDD 患者组中潜在有害细菌的发生率增加，与 密切相关： ( < 0.01)， ( < 0.05)，和 ( < 0.001)。另一方面，NDD 组患者中常见共生细菌的多样性显著降低。在较低数量的患者中检测到或在一些 NDD 患者中甚至不存在 ( < 0.05)， ( < 0.01)， ( < 0.05)， ( < 0.01)和 sp.。此外，丁酸产生菌 ( < 0.01)， ( < 0.05)和 ( = 0.07)在 NDD 患者组中较少。与此一致，粪便短链脂肪酸（SCFA）的水平也被确定。尽管 NDD 患者与对照组之间的 SCFA 水平没有显著差异，但在通用引物获得的 rDNA 扩增子数量与丙酸水平之间观察到正相关，并且在对照组中总 SCFA 和丁酸水平呈趋势。在 NDD 患者组中，这种相关性丢失，表明 NDD 患者的微生物群与健康儿童的微生物群在强 SCFA 产生菌的存在或数量上存在差异。根据范围加权丰富度指数观察到，NDD 患者组的微生物多样性明显较低。我们的研究表明，NDD 患者的肠道微生物群与健康儿童的微生物群不同。据推测，早期生命微生物组可能对广泛 NDD 患者经常观察到的胃肠道紊乱和伴随的行为问题有影响。
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