Kumar A, Dietrich S, Schneider W, Jacobson R, Downes F P, Robinson-Dunn B E, Honicky R, Smith J, Martin R
Pediatrics and Human Development, Michigan State University, East Lansing 48824-1316, USA.
Respir Med. 1997 Sep;91(8):485-92. doi: 10.1016/s0954-6111(97)90114-1.
Burkholderia cepacia isolates from patients with cystic fibrosis (CF) attending five CF centers were studied for relatedness by cellular fatty acid methyl esters (FAME) and by chromosomal DNA restriction analysis. Twenty-eight of 32 (87.5%) isolates tested were grouped in cluster group 1 based on their FAME profiles. DNA analysis revealed that 29 of 32 (90.6%) B. cepacia isolates from five CF centers had one closely related DNA pattern. To examine strain variation over a time period, FAME profiles and DNA patterns of isolates from serial cultures on seven patients from center D were studied. For four patients, all serial B. cepacia isolates belonged to a single FAME cluster group; for the remaining three patients, all serial isolates belonged to any two of the four cluster groups. On serial culture isolates, a single DNA pattern (pattern A) was found in 31 of 32 isolates demonstrating a close genetic relatedness. These data corroborate the observations that the majority of patients colonised with B. cepacia in a CF center harbor strains genetically closely related as determined by FAME profiles and DNA patterns.
对来自五个囊性纤维化（CF）中心的CF患者分离出的洋葱伯克霍尔德菌菌株，通过细胞脂肪酸甲酯（FAME）和染色体DNA限制性分析研究其亲缘关系。根据FAME谱型，32株受试菌株中有28株（87.5%）归为簇群1。DNA分析显示，来自五个CF中心的32株洋葱伯克霍尔德菌中有29株（90.6%）具有一种密切相关的DNA模式。为了研究一段时间内的菌株变异情况，对来自中心D的7名患者连续培养物中的分离株进行了FAME谱型和DNA模式研究。对于4名患者，所有连续的洋葱伯克霍尔德菌分离株都属于单个FAME簇群；对于其余3名患者，所有连续分离株属于四个簇群中的任意两个。在连续培养的分离株中，32株中有31株发现单一的DNA模式（模式A），表明存在密切的遗传相关性。这些数据证实了以下观察结果：在CF中心，大多数感染洋葱伯克霍尔德菌的患者所携带的菌株，根据FAME谱型和DNA模式确定，在遗传上密切相关。
