Kwon S Y, An C S, Liu J R, Paek K H
Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea.
Biosci Biotechnol Biochem. 1997 Sep;61(9):1613-4. doi: 10.1271/bbb.61.1613.
An antiviral protein purified from the leaves of Amaranthus viridis was named amaranthin. The in vivo antiviral activity of amaranthin was confirmed in tobacco mosaic virus (TMV) infection test on Nicotiana glutinosa leaves. The molecular mass of the amaranthin was estimated about 30 kDa by SDS-PAGE and the pI was measured as 9.8 by isoelectric focusing (IEF) analysis. Cytotoxicity of the amaranthin using in vitro translation inhibition assay was similar to that of pokeweed antiviral protein (PAP) with IC50 of 25 pM. Depurination activity (N-glycosidase activity) against animal rRNA was also confirmed.
从绿穗苋叶片中纯化得到的一种抗病毒蛋白被命名为苋红素。在对粘毛烟草叶片进行的烟草花叶病毒（TMV）感染试验中，证实了苋红素的体内抗病毒活性。通过SDS-PAGE估计苋红素的分子量约为30 kDa，通过等电聚焦（IEF）分析测得其pI为9.8。使用体外翻译抑制试验检测，苋红素的细胞毒性与商陆抗病毒蛋白（PAP）相似，IC50为25 pM。还证实了其对动物rRNA的脱嘌呤活性（N-糖苷酶活性）。
