Park J M, Kang S G, Pih K T, Jang H J, Piao H L, Yoon H W, Cho M J, Hwang I
Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Chinju, Korea.
Plant Physiol. 1997 Oct;115(2):763-71. doi: 10.1104/pp.115.2.763.
Dynamin, a GTP-binding protein, is involved in endocytosis in animal cells. We found that a dynamin-like protein, ADL1, is present in multiple forms in Arabidopsis leaf tissue. Subcellular fractionation experiments, together with gel-filtration and nondenaturing-gel electrophoresis revealed that most of ADL1 is present as a high-molecular-mass complex of 400 to 600 kD in the membrane or pellet fraction, whereas ADL1 is present in the soluble fraction as a monomer. The subcellular distribution of ADL1 is affected by various agents such as Ca2+, cyclosporin A, GTP, and ATP. Ca2+ increases the amount of ADL1 present in the membrane fraction, whereas cyclosporin A inhibits the membrane association. Furthermore, Ca2+ and GTP change the migration pattern of ADL1 in nondenaturing polyacrylamide gels, indicating that these chemicals influence either the complex formation and/or the conformation of the ADL1 complex. Our results demonstrate that ADL1 has characteristics that are similar to Dynamin I, which is found in animal cells. Therefore, it is possible that ADL1 is also involved in biological processes that require vesicle formation.
发动蛋白是一种结合GTP的蛋白质，参与动物细胞的内吞作用。我们发现，一种类似发动蛋白的蛋白质ADL1，在拟南芥叶片组织中以多种形式存在。亚细胞分级分离实验，结合凝胶过滤和非变性凝胶电泳表明，大多数ADL1以400至600 kD的高分子量复合物形式存在于膜或沉淀组分中，而ADL1以单体形式存在于可溶组分中。ADL1的亚细胞分布受多种因子影响，如Ca2+、环孢菌素A、GTP和ATP。Ca2+增加了膜组分中ADL1的含量，而环孢菌素A抑制膜结合。此外，Ca2+和GTP改变了ADL1在非变性聚丙烯酰胺凝胶中的迁移模式，表明这些化学物质影响ADL1复合物的复合物形成和/或构象。我们的结果表明，ADL1具有与动物细胞中发现的发动蛋白I相似的特征。因此，ADL1也有可能参与需要囊泡形成的生物学过程。
