Underwood M D, Arango V, Smith R W, Bakalian M J, Mann J J
Laboratories of Neuropharmacology, Western Psychiatric Institute and Clinic, University of Pittsburgh, Pennsylvania 15213, USA.
Neuroimage. 1993 Sep;1(2):79-86. doi: 10.1006/nimg.1993.1001.
Three-dimensional wireframe reconstructions were used to examine the relationship between the anatomical localization of electrode sites and the cerebrovascular response which was elicited by electrical stimulation of the dorsal raphe nucleus (DRN). Reconstructions of the rat brain and DRN were done from atlas plates and from Nissl-stained coronal sections (100-micron increments). Data points were entered and three-dimensional reconstructions were performed using commercially available software and a personal computer. Display of the entire brain yielded views which obscured visualization of the DRN. The data file was edited to reduce the number of contours without affecting the display resolution of the DRN. Selective display of the DRN and electronic rotation from the coronal to a sagittal view revealed a functional organization of the cerebral blood flow responses which was not apparent in two-dimensional coronal sections.
利用三维线框重建技术来研究电极部位的解剖定位与通过电刺激中缝背核（DRN）引发的脑血管反应之间的关系。大鼠脑和中缝背核的重建是根据图谱板和尼氏染色冠状切片（以100微米的增量）完成的。输入数据点，并使用商用软件和个人计算机进行三维重建。显示整个大脑得到的视图会遮挡中缝背核的可视化。编辑数据文件以减少轮廓数量，同时不影响中缝背核的显示分辨率。选择性显示中缝背核并从冠状视图进行电子旋转至矢状视图，揭示了二维冠状切片中不明显的脑血流反应功能组织。
