Arnutti P, Bunyaratavej A, Patanapanyasat K, Chuansumrit A
Department of Pediatrics, Pramongkutklao Hospital, Bangkok, Thailand.
J Med Assoc Thai. 1997 Sep;80 Suppl 1:S92-8.
The study on quality control of automated blood cell analyzers, Technicon H1 and Coulter MAXM by using three separately self-prepared control cells was extensively investigated. The three parts of control cells are pseudo-leukocyte and fixed platelets, which are fixed by glutaraldehyde, and control red cells from normal and thalassemic patients preserved and anticoagulated in CPD or CPDA-1. The Technicon H1 system was based on the principle of light scattering but the Coulter MAXM was based on the principle of electrical impedance for cell counting and measurement. The self-prepared control cells can be satisfactorily utilized as control for each system with statistically significant difference (p < 0.05) for both systems. The expired dates for control cells are different in both systems and should be determined for each system specifically. The control red cells prepared from thalassemic patients were quite satisfactorily useful as an abnormal control for both systems during this study.
使用三种分别自行制备的对照细胞对Technicon H1和Coulter MAXM全自动血细胞分析仪进行质量控制的研究得到了广泛调查。对照细胞的三个部分是经戊二醛固定的假白细胞和固定血小板，以及来自正常和地中海贫血患者的对照红细胞，这些红细胞保存在CPD或CPDA-1中并进行抗凝处理。Technicon H1系统基于光散射原理，而Coulter MAXM基于电阻抗原理进行细胞计数和测量。自行制备的对照细胞可令人满意地用作每个系统的对照，两个系统均具有统计学显著差异（p < 0.05）。两个系统中对照细胞的有效期不同，应针对每个系统具体确定。在本研究中，由地中海贫血患者制备的对照红细胞作为两个系统的异常对照相当令人满意。
