Kajiya H, Tanaka N, Inazumi T, Seyama Y, Tajima S, Ishibashi A
Department of Dermatology, National Defense Medical College, Saitama, Japan.
J Invest Dermatol. 1997 Nov;109(5):641-4. doi: 10.1111/1523-1747.ep12337639.
We detected elastin mRNA in cultured normal human keratinocytes by RNase protection assay. The content of elastin mRNA was estimated at approximately one-twentieth of that of cultured skin fibroblasts. Tropoelastin polypeptide with a molecular weight of 68 kDa was detected in the preparation of culture medium of normal human keratinocytes by western blot assays using anti-tropoelastin antibody. Immunohistochemical studies also demonstrated positive staining in cultured normal human keratinocytes as well as in skin fibroblasts. The expression of elastin by normal human keratinocytes was found to reach a maximum level at the quiescent phase of keratinocyte growth. When normal human keratinocytes were cultured on tropoelastin-coated dishes, their growth potential was greatly suppressed compared with other matrix protein-coated dishes. These results suggest that cultured normal human keratinocytes can actively synthesize elastin and that keratinocyte elastin may act as a growth-regulator for keratinocytes.
我们通过核糖核酸酶保护分析法在培养的正常人角质形成细胞中检测到了弹性蛋白信使核糖核酸。弹性蛋白信使核糖核酸的含量估计约为培养的皮肤成纤维细胞的二十分之一。使用抗原弹性蛋白抗体的蛋白质免疫印迹分析在正常人角质形成细胞的培养基制备物中检测到了分子量为68 kDa的原弹性蛋白多肽。免疫组织化学研究还表明，在培养的正常人角质形成细胞以及皮肤成纤维细胞中均有阳性染色。发现正常人角质形成细胞中弹性蛋白的表达在角质形成细胞生长的静止期达到最高水平。当正常人角质形成细胞在原弹性蛋白包被的培养皿上培养时，与其他基质蛋白包被的培养皿相比，它们的生长潜力受到极大抑制。这些结果表明，培养的正常人角质形成细胞可以积极合成弹性蛋白，并且角质形成细胞弹性蛋白可能作为角质形成细胞的生长调节剂。
